Acetyl-p53 (K372) Polyclonal Antibody, 100ug, (ATB-K0036)

Description

Background:

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013]

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.

Product datasheet:

Overview
Product Description	Acetyl-p53 (K372) Polyclonal Antibody, 100ug, (ATB-K0036)
Image
Species Reactivities	Human
Immunogen	Synthesized peptide derived from the human p53 around the acetylation site of K372.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Isotype	IGg

References:

1. Serum P53 Abs in HCC patients with viral hepatitis – type C. EI-Emshaty HM, et al. Hepatogastroenterology, 2014 Sep. PMID 25436364
2. Impact of G-quadruplex structures and intronic polymorphisms rs17878362 and rs1642785 on basal and ionizing radiation-induced expression of alternative p53 transcripts. Perriaud L, et al. Carcinogenesis, 2014 Dec. PMID 25269805
3. The role of tumor suppressor p53 in the antioxidant defense and metabolism. Budanov AV. Subcell Biochem, 2014. PMID 25201203 Free PMC Article
4. Involvement of p53 in the repair of DNA double strand breaks: multifaceted Roles of p53 in homologous recombination repair (HRR) and non-homologous end joining (NHEJ). Menon V, et al. Subcell Biochem, 2014. PMID 25201202 Free PMC Article
5. Mechanisms of mutant p53 stabilization in cancer. Frum RA, et al. Subcell Biochem, 2014. PMID 25201195
6. Human p53 cellular tumor antigen: cDNA sequence and expression in COS cells.
   Zakut-Houri R., Bienz-Tadmor B., Givol D., Oren M.
   EMBO J. 4:1251-1255(1985) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
7. Characterization of the human p53 gene.
   Lamb P., Crawford L.
   Mol. Cell. Biol. 6:1379-1385(1986) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), VARIANT GLY-76.
8. Molecular cloning and in vitro expression of a cDNA clone for human cellular tumor antigen p53.
   Harlow E., Williamson N.M., Ralston R., Helfman D.M., Adams T.E.
   Mol. Cell. Biol. 5:1601-1610(1985) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
9. Molecular basis for heterogeneity of the human p53 protein.
   Harris N., Brill E., Shohat O., Prokocimer M., Wolf D., Arai N., Rotter V.
   Mol. Cell. Biol. 6:4650-4656(1986) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
10. A variation in the structure of the protein-coding region of the human p53 gene.
    Buchman V.L., Chumakov P.M., Ninkina N.N., Samarina O.P., Georgiev G.P.
    Gene 70:245-252(1988) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].