Phospho-Bcl-x (T47) Polyclonal Antibody, 100µg, (ATB-P0035)

Description

Background:

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator. [provided by RefSeq, Jul 2008]

Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.

Product datasheet:

Overview
Product Description	Phospho-Bcl-x (T47) Polyclonal Antibody, 100µg, (ATB-P0035)
Image
Species Reactivities	Human,Mouse,Rat
Immunogen	Synthesized peptide derived from human Bcl-x around the phosphorylation site of T47.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Clonality	Polyclonal

References:

1. PI3K/Akt regulates survival during differentiation of human macrophages by maintaining NF-κB-dependent expression of antiapoptotic Bcl-xL. Busca A, et al. J Leukoc Biol, 2014 Dec. PMID 25225677
2. Bcl-xL controls a switch between cell death modes during mitotic arrest. Bah N, et al. Cell Death Dis, 2014 Jun 12. PMID 24922075
3. Gain of 20q11.21 in human embryonic stem cells improves cell survival by increased expression of Bcl-xL. Nguyen HT, et al. Mol Hum Reprod, 2014 Feb. PMID 24217388
4. Dynamics of Bcl-xL in water and membrane: molecular simulations. Maity A, et al. PLoS One, 2013. PMID 24116174 Free PMC Article
5. Gene-expression differences in peripheral blood between lithium responders and non-responders in the Lithium Treatment-Moderate dose Use Study (LiTMUS). Beech RD, et al. Pharmacogenomics J, 2014 Apr. PMID 23670706
6. bcl-x, a bcl-2-related gene that functions as a dominant regulator of apoptotic cell death.
   Boise L.H., Gonzalez-Garcia M., Postema C.E., Ding L., Lindsten T., Turka L.A., Mao X., Nunez G., Thompson C.B.
   Cell 74:597-608(1993) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS BCL-X(L) AND BCL-X(S)).
7. Identification of a human cDNA encoding a novel Bcl-x isoform.
   Ban J., Eckhart L., Weninger W., Mildner M., Tschachler E.
   Biochem. Biophys. Res. Commun. 248:147-152(1998) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BCL-X(BETA)), INTERACTION WITH BAX.
8. Inohara N., Ohta S.
   Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM BCL-X(BETA)).
9. The full-ORF clone resource of the German cDNA consortium.
   Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
   BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM BCL-X(L)). Tissue: Colon carcinoma.
10. Cloning of human full-length CDSs in BD Creator(TM) system donor vector.
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM BCL-X(L)).