Phospho-Bim (S59) Polyclonal Antibody, 100µg, (ATB-P1065)

Description

Background:

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family and to act as an apoptotic activator. The expression of this gene can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Transgenic studies of the mouse counterpart suggested that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection. Several alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Jun 2013]

Induces apoptosis and anoikis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than isoform BimEL, isoform BimL and isoform BimS. Isoform Bim-gamma induces apoptosis. Isoform Bim-alpha3 induces apoptosis possibly through a caspase-mediated pathway. Isoform BimAC and isoform BimABC lack the ability to induce apoptosis.

Product datasheet:

References:

1. A Bim-targeting strategy overcomes adaptive bortezomib resistance in myeloma through a novel link between autophagy and apoptosis. Chen S, et al. Blood, 2014 Oct 23. PMID 25208888
2. The Bim deletion polymorphism clinical profile and its relation with tyrosine kinase inhibitor resistance in Chinese patients with non-small cell lung cancer. Zhao M, et al. Cancer, 2014 Aug 1. PMID 24737648
3. Clinical significance of BIM deletion polymorphism in non-small-cell lung cancer with epidermal growth factor receptor mutation. Isobe K, et al. J Thorac Oncol, 2014 Apr. PMID 24736070 Free PMC Article
4. c-Jun N-terminal kinase – c-Jun pathway transactivates Bim to promote osteoarthritis. Ye Z, et al. Can J Physiol Pharmacol, 2014 Feb. PMID 24502636
5. BIMEL is a key effector molecule in oxidative stress-mediated apoptosis in acute myeloid leukemia cells when combined with arsenic trioxide and buthionine sulfoximine. Tanaka Y, et al. BMC Cancer, 2014 Jan 15. PMID 24428916 Free PMC Article
6. Bim: a novel member of the Bcl-2 family that promotes apoptosis.
   O’Connor L., Strasser A., O’Reilly L.A., Hausmann G., Adams J.M., Cory S., Huang D.C.S.
   EMBO J. 17:384-395(1998) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS BIMEL AND BIML), FUNCTION. Tissue: Peripheral blood and Spleen.
7. Molecular cloning and characterization of six novel isoforms of human Bim, a member of the proapoptotic Bcl-2 family.
   Mami U., Miyashita T., Shikama Y., Tadokoro K., Yamada M.
   FEBS Lett. 509:135-141(2001) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS BIM-ALPHA1; BIM-ALPHA2; BIM-BETA1; BIM-BETA2; BIM-BETA3 AND BIM-BETA4), FUNCTION (ISOFORMS BIM-ALPHA1 AND BIM-ALPHA2), SUBCELLULAR LOCATION.
8. Identification and characterization of Bimgamma, a novel proapoptotic BH3-only splice variant of Bim.
   Liu J.-W., Chandra D., Tang S.H., Chopra D., Tang D.G.
   Cancer Res. 62:2976-2981(2002) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BIM-GAMMA), FUNCTION (ISOFORM BIM-GAMMA), SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
9. Identification of novel isoforms of the BH3 domain protein Bim which directly activate Bax to trigger apoptosis.
   Marani M., Tenev T., Hancock D., Downward J., Lemoine N.R.
   Mol. Cell. Biol. 22:3577-3589(2002) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS BIMEL; BIML; BIMS; BIM-ALPHA1; BIM-ALPHA2; BIM-ALPHA3; BIMA; BIMABC AND BIMAC), ALTERNATIVE SPLICING, FUNCTION, SUBUNIT, MUTAGENESIS OF GLY-156 AND ASN-160. Tissue: Embryonic kidney and Ovarian cancer.
10. Over-expression of Bim alpha3, a novel isoform of human Bim, result in cell apoptosis.
    Chen J.Z., Ji C.N., Gu S.H., Li J.X., Zhao E.P., Huang Y., Huang L., Ying K., Xie Y., Mao Y.M.
    Int. J. Biochem. Cell Biol. 36:1554-1561(2004) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS BIMS AND BIM-ALPHA3), FUNCTION (ISOFORM BIM-ALPHA3).