Phospho-BLNK (Y84) Polyclonal Antibody, 100µg, (ATB-P0802)

Description

Background:

This gene encodes a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2012]

Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca²⁺ mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis.

Product datasheet:

Overview
Product Description	Phospho-BLNK (Y84) Polyclonal Antibody, 100µg, (ATB-P0802)
Image
Species Reactivities	Human,Mouse,Rat
Immunogen	Synthesized peptide derived from human BLNK around the phosphorylation site of Y84.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Clonality	Polyclonal

References:

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2. B-cell linker protein prevents aneuploidy by inhibiting cytokinesis. Kamino H, et al. Cancer Sci, 2008 Dec. PMID 19018766
3. SLP65 deficiency results in perpetual V(D)J recombinase activity in pre-B-lymphoblastic leukemia and B-cell lymphoma cells. Sprangers M, et al. Oncogene, 2006 Aug 24. PMID 16636677
4. Cutting edge: feed-forward activation of phospholipase Cγ2 via C2 domain-mediated binding to SLP65. Engelke M, et al. J Immunol, 2013 Dec 1. PMID 24166973
5. RUNX1 mutations in cytogenetically normal acute myeloid leukemia are associated with a poor prognosis and up-regulation of lymphoid genes. Greif PA, et al. Haematologica, 2012 Dec. PMID 22689681 Free PMC Article
6. BLNK: a central linker protein in B cell activation.
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   Immunity 9:93-103(1998) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), PROTEIN SEQUENCE OF 7-20; 140-146; 237-248; 250-257; 366-373 AND 392-405, FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH PLCG1; VAV1; GRB2 AND NCK1, ALTERNATIVE SPLICING, PHOSPHORYLATION, MUTAGENESIS OF TYR-72; TYR-84; TYR-96 AND TYR-178.
7. An essential role for BLNK in human B cell development.
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   Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

10. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: B-cell.