Phospho-Caspase-9 (Y153) Polyclonal Antibody, 100µg, (ATB-P0542)

Description

Background:

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).

Product datasheet:

Overview
Product Description	Phospho-Caspase-9 (Y153) Polyclonal Antibody, 100µg, (ATB-P0542)
Image
Species Reactivities	Human
Immunogen	Synthesized peptide derived from human Caspase-9 around the phosphorylation site of Y153.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Clonality	Polyclonal

References:

1. Association of caspase9 promoter polymorphisms with the susceptibility of AML in south Indian subjects. Cingeetham A, et al. Tumour Biol, 2014 Sep. PMID 24879622
2. Silica and double-stranded RNA synergistically induce bronchial epithelial apoptosis and airway inflammation. Unno H, et al. Am J Respir Cell Mol Biol, 2014 Sep. PMID 24661197
3. Apoptotic block in colon cancer cells may be rectified by lentivirus mediated overexpression of caspase-9. Xu D, et al. Acta Gastroenterol Belg, 2013 Dec. PMID 24592539
4. Induction of caspase-9, biochemical assessment and morphological changes caused by apoptosis in cancer cells treated with goniothalamin extracted from Goniothalamus macrophyllus. Alabsi AM, et al. Asian Pac J Cancer Prev, 2013. PMID 24377517
5. Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Kearns NA, et al. Development, 2014 Jan. PMID 24346702 Free PMC Article
6. ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B.
   Duan H., Orth K., Chinnaiyan A.M., Poirier G.G., Froelich C.J., He W.-W., Dixit V.M.
   J. Biol. Chem. 271:16720-16724(1996) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS VAL-28 AND ARG-221.
7. The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32.
   Srinivasula S.M., Fernandes-Alnemri T., Zangrilli J., Robertson N., Armstrong R.C., Wang L., Trapani J.A., Tomaselli K.J., Litwack G., Alnemri E.S.
   J. Biol. Chem. 271:27099-27106(1996) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEOLYTIC PROCESSING. Tissue: T-cell.
8. Genomic organization of the human caspase-9 gene on chromosome 1p36.1-p36.3.
   Hadano S., Nasir J., Nichol K., Rasper D.M., Vaillancourt J.P., Sherer S.W., Beatty B.G., Ikeda J.E., Nicholson D.W., Hayden M.R.
   Mamm. Genome 10:757-760(1999) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
9. Identification of an endogenous dominant-negative short isoform of caspase-9 that can regulate apoptosis.
   Srinivasula S.M., Ahmad M., Guo Y., Zhan Y., Lazebnik Y., Fernandes-Alnemri T., Alnemri E.S.
   Cancer Res. 59:999-1002(1999) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
10. Molecular cloning and sequencing of a cDNA predicting an alternative form of pro-caspase-9 from human gastric cancer cell lines.
    Izawa M., Mori T., Ito H., Sairenji T.
    Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Stomach cancer.