Phospho-CD31 (Y713) Polyclonal Antibody, 100µg, (ATB-P0530)

Description

Background:

The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]

Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting ‘detachment’ signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell’s active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).

Product datasheet:

Overview
Product Description	Phospho-CD31 (Y713) Polyclonal Antibody, 100µg, (ATB-P0530)
Image
Species Reactivities	Human,Mouse
Immunogen	Synthesized peptide derived from human CD31 around the phosphorylation site of Y713.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Clonality	Polyclonal

References:

1. Regulation of endothelial cell barrier function by antibody-driven affinity modulation of platelet endothelial cell adhesion molecule-1 (PECAM-1). Mei H, et al. J Biol Chem, 2014 Jul 25. PMID 24936065
2. Platelet endothelial cell adhesion molecule-1, a putative receptor for the adhesion of Streptococcus pneumoniae to the vascular endothelium of the blood-brain barrier. Iovino F, et al. Infect Immun, 2014 Sep. PMID 24914219 Free PMC Article
3. Expression of CD31/PECAM-1 (platelet endothelial cell adhesion molecule 1) by blastic plasmacytoid dendritic cell neoplasms. Salva KA, et al. JAMA Dermatol, 2014 Jan. PMID 24225899 Free PMC Article
4. CD31 expression on peripheral blood stem cells predicts both early neutrophil and platelet engraftments. Donmez A, et al. Transfus Apher Sci, 2013 Oct. PMID 24025427
5. Association of single nucleotide polymorphisms in the gene encoding platelet endothelial cell adhesion molecule-1 with the risk of myocardial infarction: a systematic review and meta-analysis. Sahebkar A, et al. Thromb Res, 2013 Aug. PMID 23906939
6. Molecular cloning of CD31, a putative intercellular adhesion molecule closely related to carcinoembryonic antigen.
   Simmons D.L., Walker C., Power C., Pigott R.
   J. Exp. Med. 171:2147-2152(1990) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
7. Molecular characterization and functional analysis of the leukocyte surface protein CD31.
   Stockinger H., Gadd S.J., Eher R., Majdic O., Kasinrek W., Schreiber W., Strass B., Schnabl E., Knapp W.
   J. Immunol. 145:3889-3897(1990) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG), VARIANTS VAL-125; ASN-563 AND GLY-670.
8. PECAM-1 (CD31) cloning and relation to adhesion molecules of the immunoglobulin gene superfamily.
   Newman P.J., Berndt M.C., Gorski J., White J.C. II, Lyman S., Paddock C., Muller W.A.
   Science 247:1219-1222(1990) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
9. Molecular and cellular properties of PECAM-1 (endoCAM/CD31): a novel vascular cell-cell adhesion molecule.
   Albelda S.M., Muller W.A., Buck C.A., Newman P.J.
   J. Cell Biol. 114:1059-1068(1991) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
10. Organization of the gene for human platelet/endothelial cell adhesion molecule-1 shows alternatively spliced isoforms and a functionally complex cytoplasmic domain.
    Kirschbaum N.E., Gumina R.J., Newman P.J.
    Blood 84:4028-4037(1994) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM LONG), VARIANT ASN-563.