Phospho-EGFR (Y1069) Polyclonal Antibody, 100µg, (ATB-P0355)

Description

Background:

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.

Product datasheet:

Overview
Product Description	Phospho-EGFR (Y1069) Polyclonal Antibody, 100µg, (ATB-P0355)
Image
Species Reactivities	Human,Mouse,Rat,Monkey
Immunogen	Synthesized peptide derived from human EGFR around the phosphorylation site of Y1069.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Clonality	Polyclonal

References:

1. Quantitative in vivo immunohistochemistry of epidermal growth factor receptor using a receptor concentration imaging approach. Samkoe KS, et al. Cancer Res, 2014 Dec 15. PMID 25344226
2. Head and neck squamous cell carcinomas do not express EGFRvIII. Melchers LJ, et al. Int J Radiat Oncol Biol Phys, 2014 Oct 1. PMID 25304797
3. Non-invasive detection of EGFR deletion at exon 19 in non-small cell lung cancer by real time diagnostic. Sun H, et al. Clin Lab, 2014. PMID 25291948
4. Associations between epidermal growth factor receptor gene mutation and serum tumor markers in advanced lung adenocarcinomas: a retrospective study. Pan YQ, et al. Chin Med Sci J, 2014 Sep. PMID 25264883
5. Epidermal growth factor receptor (EGFR) mutation testing, from bench to practice: a single institute experience. Shikhrakab H, et al. Ir Med J, 2014 Jul-Aug. PMID 25226713
6. Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells.
   Ullrich A., Coussens L., Hayflick J.S., Dull T.J., Gray A., Tam A.W., Lee J., Yarden Y., Libermann T.A., Schlessinger J., Downward J., Mayes E.L.V., Whittle N., Waterfield M.D., Seeburg P.H.
   Nature 309:418-425(1984) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
7. Possible role of variant RNA transcripts in the regulation of epidermal growth factor receptor expression in human placenta.
   Ilekis J.V., Stark B.C., Scoccia B.
   Mol. Reprod. Dev. 41:149-156(1995) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Placenta.
8. A 1.8 kb alternative transcript from the human epidermal growth factor receptor gene encodes a truncated form of the receptor.
   Reiter J.L., Maihle N.J.
   Nucleic Acids Res. 24:4050-4056(1996) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 2). Tissue: Placenta.
9. Expression of a truncated epidermal growth factor receptor-like protein (TEGFR) in ovarian cancer.
   Ilekis J.V., Gariti J., Niederberger C., Scoccia B.
   Gynecol. Oncol. 65:36-41(1997) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Placenta.
10. Comparative genomic sequence analysis and isolation of human and mouse alternative EGFR transcripts encoding truncated receptor isoforms.
    Reiter J.L., Threadgill D.W., Eley G.D., Strunk K.E., Danielsen A.J., Schehl Sinclair C., Pearsall R.S., Green P.J., Yee D., Lampland A.L., Balasubramaniam S., Crossley T.D., Magnuson T.R., James C.D., Maihle N.J.
    Genomics 71:1-20(2001) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 3 AND 4). Tissue: Placenta.