Phospho-GluR4 (S862) Polyclonal Antibody, 100µg, (ATB-P0740)

Description

Background:

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors, and is subject to RNA editing (AGA->GGA; R->G). Alternative splicing of this gene results in transcript variants encoding different isoforms, which may vary in their signal transduction properties. Some haplotypes of this gene show a positive association with schizophrenia. [provided by RefSeq, Jul 2008]

Receptor for glutamate that functions as ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.

Product datasheet:

Overview
Product Description	Phospho-GluR4 (S862) Polyclonal Antibody, 100µg, (ATB-P0740)
Image
Species Reactivities	Human,Mouse,Rat
Immunogen	Synthesized peptide derived from human GluR4 around the phosphorylation site of S862.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Clonality	Polyclonal

References:

1. Kinetic mechanism of channel opening of the GluRDflip AMPA receptor. Li G, et al. Biochemistry, 2005 Apr 19. PMID 15823042
2. No genetic association between polymorphisms in the AMPA receptor subunit GluR4 gene (GRIA4) and schizophrenia in the Chinese population. Guo S, et al. Neurosci Lett, 2004 Oct 14. PMID 15450689
3. GluR4c, an alternative splicing isoform of GluR4, is abundantly expressed in the adult human brain. Kawahara Y, et al. Brain Res Mol Brain Res, 2004 Aug 23. PMID 15306133
4. Alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor channels lacking the N-terminal domain. Pasternack A, et al. J Biol Chem, 2002 Dec 20. PMID 12393905
5. Cloning, expression and pharmacological characterization of a human glutamate receptor: hGluR4. Fletcher EJ, et al. Receptors Channels, 1995. PMID 8589990
6. Cloning, expression and pharmacological characterization of a human glutamate receptor: hGluR4.
   Fletcher E.J., Nutt S.L., Hoo K.H., Elliott C.E., Korczak B., McWhinnie E.A., Kamboj R.K.
   Recept. Channels 3:21-31(1995) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Brain.
7. Human chromosome 11 DNA sequence and analysis including novel gene identification.
   Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G.
   , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.
   Nature 440:497-500(2006) [PubMed] [Europe PMC]

   Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

8. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
   The MGC Project Team
   Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
9. Hippocampal AMPA receptor gating controlled by both TARP and cornichon proteins.
   Kato A.S., Gill M.B., Ho M.T., Yu H., Tu Y., Siuda E.R., Wang H., Qian Y.W., Nisenbaum E.S., Tomita S., Bredt D.S.
   Neuron 68:1082-1096(2010) [PubMed] [Europe PMC] Cited for: FUNCTION.