Phospho-Hrs (Y334) Polyclonal Antibody, 100µg, (ATB-P0935)

Description

Background:

The protein encoded by this gene regulates endosomal sorting and plays a critical role in the recycling and degradation of membrane receptors. The encoded protein sorts monoubiquitinated membrane proteins into the multivesicular body, targeting these proteins for lysosome-dependent degradation. [provided by RefSeq, Dec 2010]

Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation.

Product datasheet:

Overview
Product Description	Phospho-Hrs (Y334) Polyclonal Antibody, 100µg, (ATB-P0935)
Image
Species Reactivities	Human,Mouse,Rat
Immunogen	Synthesized peptide derived from human Hrs around the phosphorylation site of Y334.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Clonality	Polyclonal

References:

1. An essential role of Hrs/Vps27 in endosomal cholesterol trafficking. Du X, et al. Cell Rep, 2012 Jan 26. PMID 22832105
2. Regulation of hepatitis C virus secretion by the Hrs-dependent exosomal pathway. Tamai K, et al. Virology, 2012 Jan 20. PMID 22138215
3. Clinical value of plasma hepatocyte growth factor measurement for the diagnosis of periampullary cancer and prognosis after pancreaticoduodenectomy. Barakat O, et al. J Surg Oncol, 2010 Dec 1. PMID 20812348
4. ESCRT-I function is required for Tyrp1 transport from early endosomes to the melanosome limiting membrane. Truschel ST, et al. Traffic, 2009 Sep. PMID 19624486 Free PMC Article
5. Essential role of Hrs in a recycling mechanism mediating functional resensitization of cell signaling. Hanyaloglu AC, et al. EMBO J, 2005 Jul 6. PMID 15944737 Free PMC Article
6. Hrs is associated with STAM, a signal-transducing adaptor molecule. Its suppressive effect on cytokine-induced cell growth.
   Asao H., Sasaki Y., Arita T., Tanaka N., Endo K., Kasai H., Takeshita T., Endo Y., Fujita T., Sugamura K.
   J. Biol. Chem. 272:32785-32791(1997) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, INTERACTION WITH STAM.
7. Human Hrs, a tyrosine kinase substrate in growth factor-stimulated cells: cDNA cloning and mapping of the gene to chromosome 17.
   Lu L., Komada M., Kitamura N.
   Gene 213:125-132(1998) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY. Tissue: Placenta.
8. The neurofibromatosis 2 tumor suppressor protein interacts with hepatocyte growth factor-regulated tyrosine kinase substrate.
   Scoles D.R., Huynh D.P., Chen M.S., Burke S.P., Gutmann D.H., Pulst S.-M.
   Hum. Mol. Genet. 9:1567-1574(2000) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), DOMAIN, INTERACTION WITH NF2, SUBCELLULAR LOCATION. Tissue: Brain.
9. Cloning of human full-length CDSs in BD Creator(TM) system donor vector.
   Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
   Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
10. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Skin.