Phospho-IκB-α (Y305) Polyclonal Antibody, 100µg, (ATB-P0752)

Description

Background:

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.

Product datasheet:

Overview
Product Description	Phospho-IκB-α (Y305) Polyclonal Antibody, 100µg, (ATB-P0752)
Image
Species Reactivities	Human,Mouse,Rat,Monkey
Immunogen	Synthesized peptide derived from human IκB-α around the phosphorylation site of Y305.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Clonality	Polyclonal

References:

1. MiR-196a promotes pancreatic cancer progression by targeting nuclear factor kappa-B-inhibitor alpha. Huang F, et al. PLoS One, 2014. PMID 24504166 Free PMC Article
2. Inhibitor IκBα promoter functional polymorphisms in patients with multiple sclerosis. Balood M, et al. Mol Biol Rep, 2014 Feb. PMID 24368589
3. Frequency of NFKBIA deletions is low in glioblastomas and skewed in glioblastoma neurospheres. Patanè M, et al. Mol Cancer, 2013 Dec 11. PMID 24330732 Free PMC Article
4. Intracellular NF-kB-decrease and IKBα increase in human macrophages following CTLA4-Ig treatment. Cutolo M, et al. Clin Exp Rheumatol, 2013 Nov-Dec. PMID 24295474
5. Long-term incubation with proteasome inhibitors (PIs) induces IκBα degradation via the lysosomal pathway in an IκB kinase (IKK)-dependent and IKK-independent manner. Lee KH, et al. J Biol Chem, 2013 Nov 8. PMID 24085292 Free PMC Article
6. Characterization of an immediate-early gene induced in adherent monocytes that encodes I kappa B-like activity.
   Haskill S., Beg A.A., Tompkins S.M., Morris J.S., Yurochko A.D., Sampson-Johannes A., Mondal K., Ralph P., Baldwin A.S. Jr.
   Cell 65:1281-1289(1991) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Monocyte.
7. Clonal deleterious mutations in the IkappaB alpha gene in the malignant cells in Hodgkin’s lymphoma.
   Jungnickel B., Staratschek-Jox A., Braeuninger A., Spieker T., Wolf J., Diehl V., Hansmann M.-L., Rajewsky K., Kueppers R.
   J. Exp. Med. 191:395-402(2000) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Lymph node.
8. Homo sapiens IkBa mRNA.
   Liu B., Huang A.
   Submitted (APR-2001) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [MRNA].
9. Cloning of human full-length CDSs in BD Creator(TM) system donor vector.
   Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
   Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
10. SeattleSNPs variation discovery resource
    Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].