Phospho-IKKα/β (S180/181) Polyclonal Antibody, 100µg, (ATB-P0142)

Description

Background:

This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008]

Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, TAX1BP1, and the E3 ligases ITCH and RNF11). Therefore, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. In turn, these complexes regulate genes encoding molecules involved in B-cell survival and lymphoid organogenesis. Participates also in the negative feedback of the non-canonical NF-kappa-B signaling pathway by phosphorylating and destabilizing MAP3K14/NIK. Within the nucleus, phosphorylates CREBBP and consequently increases both its transcriptional and histone acetyltransferase activities. Modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histones H3 at ‘Ser-10’ that are subsequently acetylated at ‘Lys-14’ by CREBBP. Additionally, phosphorylates the CREBBP-interacting protein NCOA3.

Product datasheet:

Overview
Product Description	Phospho-IKKα/β (S180/181) Polyclonal Antibody, 100µg, (ATB-P0142)
Image
Species Reactivities	Human,Mouse,Rat
Immunogen	Synthesized peptide derived from human IKKα/β around the phosphorylation site of S180/181.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Clonality	Polyclonal

References:

1. Ikappa B kinase alpha involvement in the development of nasopharyngeal carcinoma through a NF-κB-independent and ERK-dependent pathway. Xie Y, et al. Oral Oncol, 2013 Dec. PMID 24075781
2. IκB kinase complex (IKK) triggers detachment-induced autophagy in mammary epithelial cells independently of the PI3K-AKT-MTORC1 pathway. Chen N, et al. Autophagy, 2013 Aug. PMID 23778976 Free PMC Article
3. Hepatitis C virus infection activates an innate pathway involving IKK-α in lipogenesis and viral assembly. Li Q, et al. Nat Med, 2013 Jun. PMID 23708292 Free PMC Article
4. Novel IKKβ inhibitors discovery based on the co-crystal structure by using binding-conformation-based and ligand-based method. Huang JJ, et al. Eur J Med Chem, 2013 May. PMID 23501112
5. Beyond NF-κB activation: nuclear functions of IκB kinase α. Huang WC, et al. J Biomed Sci, 2013 Jan 23. PMID 23343355 Free PMC Article
6. Identification and characterization of an IkappaB kinase.
   Regnier C.H., Song H.Y., Gao X., Goeddel D.V., Cao Z., Rothe M.
   Cell 90:373-383(1997) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF LYS-44, VARIANT ILE-268. Tissue: T-cell.
7. A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB.
   DiDonato J.A., Hayakawa M., Rothwarf D.M., Zandi E., Karin M.
   Nature 388:548-554(1997) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, VARIANT ILE-268.
8. IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation.
   Mercurio F., Zhu H., Murray B.W., Shevchenko A., Bennett B.L., Li J.W., Young D.B., Barbosa M., Mann M., Manning A., Rao A.
   Science 278:860-866(1997) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, MUTAGENESIS OF LYS-44 AND SER-176. Tissue: Cervix carcinoma.
9. IkappaB kinase-alpha and -beta genes are coexpressed in adult and embryonic tissues but localized to different human chromosomes.
   Hu M.C.-T., Wang Y.-P.
   Gene 222:31-40(1998) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ILE-268. Tissue: Heart.
10. SeattleSNPs variation discovery resource
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].