Phospho-JAK2 (Y1007) Polyclonal Antibody, 100µg, (ATB-P0155)

Description

Background:

This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. [provided by RefSeq, Jul 2008]

Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins. Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation. Plays a role in cell cycle by phosphorylating CDKN1B. Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of ‘Tyr-41’ of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin.

Product datasheet:

Overview
Product Description	Phospho-JAK2 (Y1007) Polyclonal Antibody, 100µg, (ATB-P0155)
Image
Species Reactivities	Human,Mouse,Rat
Immunogen	Synthesized peptide derived from human JAK2 around the phosphorylation site of Y1007.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Clonality	Polyclonal

References:

1. Distinct driver mutation profiles of childhood and adolescent essential thrombocythemia. Langabeer SE, et al. Pediatr Blood Cancer, 2015 Jan. PMID 25132652
2. Interferon α-2b gains high sustained response therapy for advanced essential thrombocythemia and polycythemia vera with JAK2V617F positive mutation. Huang BT, et al. Leuk Res, 2014 Oct. PMID 25069759
3. The role of serum erythropoietin level and JAK2 V617F allele burden in the diagnosis of polycythaemia vera. Ancochea A, et al. Br J Haematol, 2014 Nov. PMID 25040297
4. The JAK2 46/1 haplotype (GGCC) in myeloproliferative neoplasms and splanchnic vein thrombosis: a pooled analysis of 26 observational studies. Li SL, et al. Ann Hematol, 2014 Nov. PMID 25015051
5. Molecular basis for pseudokinase-dependent autoinhibition of JAK2 tyrosine kinase. Shan Y, et al. Nat Struct Mol Biol, 2014 Jul. PMID 24918548
6. Cloning and characterization of human Jak-2 kinase: high mRNA expression in immune cells and muscle tissue.
   Saltzman A., Stone M., Franks C., Searfoss G., Munro R., Jaye M., Ivashchenko Y.
   Biochem. Biophys. Res. Commun. 246:627-633(1998) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
7. Cloning and characterization of the human homolog of mouse Jak2.
   Dalal I., Arpaia E., Dadi H., Kulkarni S., Squire J., Roifman C.M.
   Blood 91:844-851(1998) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
8. Fusion of TEL, the ETS-variant gene 6 (ETV6), to the receptor-associated kinase JAK2 as a result of t(9;12) in a lymphoid and t(9;15;12) in a myeloid leukemia.
   Peeters P., Raynaud S.D., Cools J., Wlodarska I., Grosgeorge J., Philip P., Monpoux F., Van Rompaey L., Baens M., Van Den Berghe H., Marynen P.
   Blood 90:2535-2540(1997) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHROMOSOMAL TRANSLOCATION WITH ETV6.
9. DNA sequence and analysis of human chromosome 9.
   Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L.
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   Nature 429:369-374(2004) [PubMed] [Europe PMC]

   Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

10. The human homologue of the yeast proteins Skb1 and Hsl7p interacts with Jak kinases and contains protein methyltransferase activity.
    Pollack B.P., Kotenko S.V., He W., Izotova L.S., Barnoski B.L., Pestka S.
    J. Biol. Chem. 274:31531-31542(1999) [PubMed] [Europe PMC] Cited for: INTERACTION WITH SKB1.