Phospho-LATS1/2 (T1079/1041) Polyclonal Antibody, 100µg, (ATB-P1047)

Description

Background:

The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments. Two protein-coding transcripts and one non-protein coding transcript have been found for this gene. [provided by RefSeq, Jul 2012]

Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function.

Product datasheet:

References:

1. Underexpression of LATS1 TSG in colorectal cancer is associated with promoter hypermethylation. Wierzbicki PM, et al. World J Gastroenterol, 2013 Jul 21. PMID 23885148 Free PMC Article
2. Decreased expression of LATS1 is correlated with the progression and prognosis of glioma. Ji T, et al. J Exp Clin Cancer Res, 2012 Aug 21. PMID 22909338 Free PMC Article
3. Tumor suppressor WARTS ensures genomic integrity by regulating both mitotic progression and G1 tetraploidy checkpoint function. Iida S, et al. Oncogene, 2004 Jul 8. PMID 15122335
4. Inhibition of cell growth by conditional expression of kpm, a human homologue of Drosophila warts/lats tumor suppressor. Kamikubo Y, et al. J Biol Chem, 2003 May 16. PMID 12624101
5. Molecular alterations of h-warts/LATS1 tumor suppressor in human soft tissue sarcoma. Hisaoka M, et al. Lab Invest, 2002 Oct. PMID 12379777
6. Human homologue of the Drosophila melanogaster lats tumour suppressor modulates CDC2 activity.
   Tao W., Zhang S., Turenchalk G.S., Stewart R.A., St John M.A., Chen W., Xu T.
   Nat. Genet. 21:177-181(1999) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PHOSPHORYLATION, INTERACTION WITH CDK1. Tissue: Fetal brainImported

7. <p>Manually validated information which has been imported from another database.</p>
10. <p><a href=”/manual/evidences#ECO:0000312″>More…</a></p> Manual assertion inferred from database entriesⁱ
    EMBL:AAD16882.1