Phospho-MEK-1 (S298) Polyclonal Antibody, 100µg, (ATB-P0329)

Description

Background:

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.

Product datasheet:

Overview
Product Description	Phospho-MEK-1 (S298) Polyclonal Antibody, 100µg, (ATB-P0329)
Image
Species Reactivities	Human,Mouse,Rat
Immunogen	Synthesized peptide derived from human MEK-1 around the phosphorylation site of S298.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Clonality	Polyclonal

References:

1. High prevalence of somatic MAP2K1 mutations in BRAF V600E-negative Langerhans cell histiocytosis. Brown NA, et al. Blood, 2014 Sep 4. PMID 24982505
2. The role of MEK inhibitors in the treatment of metastatic melanoma. Grimaldi AM, et al. Curr Opin Oncol, 2014 Mar. PMID 24419498
3. Development, characterization, and reversal of acquired resistance to the MEK1 inhibitor selumetinib (AZD6244) in an in vivo model of childhood astrocytoma. Bid HK, et al. Clin Cancer Res, 2013 Dec 15. PMID 24132923 Free PMC Article
4. Substituted 3-benzylcoumarins as allosteric MEK1 inhibitors: design, synthesis and biological evaluation as antiviral agents. Wang C, et al. Molecules, 2013 May 21. PMID 23698055
5. Truncated MEK1 is required for transient activation of MAPK signalling in G2 phase cells. Pike T, et al. Cell Signal, 2013 Jun. PMID 23524336
6. Human T-cell mitogen-activated protein kinase kinases are related to yeast signal transduction kinases.
   Seger R., Seger D., Lozeman F.J., Ahn N.G., Graves L.M., Campbell J.S., Ericsson L., Harrylock M., Jensen A.M., Krebs E.G.
   J. Biol. Chem. 267:25628-25631(1992) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), PARTIAL PROTEIN SEQUENCE, TISSUE SPECIFICITY. Tissue: T-cell.
7. Cloning and characterization of two distinct human extracellular signal-regulated kinase activator kinases, MEK1 and MEK2.
   Zheng C.-F., Guan K.-L.
   J. Biol. Chem. 268:11435-11439(1993) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
8. Activation of MEK family kinases requires phosphorylation of two conserved Ser/Thr residues.
   Zheng C.-F., Guan K.-L.
   EMBO J. 13:1123-1131(1994) [PubMed] [Europe PMC] Cited for: PHOSPHORYLATION AT SER-218 AND SER-222, MUTAGENESIS.
9. Proteolytic inactivation of MAP-kinase-kinase by anthrax lethal factor.
   Duesbery N.S., Webb C.P., Leppla S.H., Gordon V.M., Klimpel K.R., Copeland T.D., Ahn N.G., Oskarsson M.K., Fukasawa K., Paull K.D., Vande Woude G.F.
   Science 280:734-737(1998) [PubMed] [Europe PMC] Cited for: CLEAVAGE BY ANTHRAX LETHAL FACTOR, PROTEIN SEQUENCE OF 9-17.
10. Susceptibility of mitogen-activated protein kinase kinase family members to proteolysis by anthrax lethal factor.
    Vitale G., Bernardi L., Napolitani G., Mock M., Montecucco C.
    Biochem. J. 352:739-745(2000) [PubMed] [Europe PMC] Cited for: CLEAVAGE BY ANTHRAX LETHAL FACTOR.