Phospho-MEK-7 (T275) Polyclonal Antibody, 100µg, (ATB-P0426)

Description

Background:

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Has a specific role in JNK signal transduction pathway activated by proinflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis.

Product datasheet:

Overview
Product Description	Phospho-MEK-7 (T275) Polyclonal Antibody, 100µg, (ATB-P0426)
Image
Species Reactivities	Human,Mouse,Rat
Immunogen	Synthesized peptide derived from human MEK-7 around the phosphorylation site of T275.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Clonality	Polyclonal

References:

1. Alpinetin suppresses proliferation of human hepatoma cells by the activation of MKK7 and elevates sensitization to cis-diammined dichloridoplatium. Tang B, et al. Oncol Rep, 2012 Apr. PMID 22159816 Free PMC Article
2. Involvement of oxidative stress in taxol-induced apoptosis in chronic myelogenous leukemia K562 cells. Meshkini A, et al. Exp Toxicol Pathol, 2012 May. PMID 21074392
3. Cloning and expression of human mitogen-activated protein kinase kinase 7gamma1. Michael L, et al. Biochem Biophys Res Commun, 2006 Mar 17. PMID 16442502
4. MKK7 is an essential component of the JNK signal transduction pathway activated by proinflammatory cytokines. Tournier C, et al. Genes Dev, 2001 Jun 1. PMID 11390361 Free PMC Article
5. Role of MEKK2-MEK5 in the regulation of TNF-alpha gene expression and MEKK2-MKK7 in the activation of c-Jun N-terminal kinase in mast cells. Chayama K, et al. Proc Natl Acad Sci U S A, 2001 Apr 10. PMID 11274363 Free PMC Article
6. Molecular cloning and characterization of human JNKK2, a novel jun NH2-terminal kinase-specific kinase.
   Wu Z., Wu J., Jacinto E., Karin M.
   Mol. Cell. Biol. 17:7407-7416(1997) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY. Tissue: Heart and Skeletal muscle.
7. Identification of c-Jun NH2-terminal protein kinase (JNK)-activating kinase 2 as an activator of JNK but not p38.
   Lu X., Nemoto S., Lin A.
   J. Biol. Chem. 272:24751-24754(1997) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, ENZYME REGULATION.
8. Human mitogen-activated protein kinase kinase 7 (MKK7) is a highly conserved c-Jun N-terminal kinase/stress-activated protein kinase (JNK/SAPK) activated by environmental stresses and physiological stimuli.
   Foltz I.N., Gerl R.E., Wieler J.S., Luckach M., Salmon R.A., Schrader J.W.
   J. Biol. Chem. 273:9344-9351(1998) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, TISSUE SPECIFICITY, VARIANT PHE-259. Tissue: Fetal kidney.
9. Cloning and expression of human mitogen-activated protein kinase kinase 7gamma1.
   Michael L., Swantek J., Robinson M.J.
   Biochem. Biophys. Res. Commun. 341:679-683(2006) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), ENZYME REGULATION, TISSUE SPECIFICITY.
10. Molecular cloning of human JNKK2 reveals a novel kinase module for c-Jun N-terminal kinase activation.
    Yang J., New L., Yong J., Han J., Su B.
    Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION IN PHOSPHORYLATION OF MAPK8/JNK1 AND MAPK9/JNK2.