Phospho-MKP-1/2 (S296/318) Polyclonal Antibody, 100µg, (ATB-P0823)

Description

Background:

The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation. [provided by RefSeq, Jul 2008]

Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both ‘Thr-183’ and ‘Tyr-185’, regulating its activity during the meiotic cell cycle.

Product datasheet:

Overview
Product Description	Phospho-MKP-1/2 (S296/318) Polyclonal Antibody, 100µg, (ATB-P0823)
Image
Species Reactivities	Human,Mouse,Rat
Immunogen	Synthesized peptide derived from human MKP-1/2 around the phosphorylation site of S296/318.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Clonality	Polyclonal

References:

1. MAP kinase phosphatase DUSP1 is overexpressed in obese humans and modulated by physical exercise. Khadir A, et al. Am J Physiol Endocrinol Metab, 2015 Jan 1. PMID 25370852
2. Roles for the mitogen-activated protein kinase (MAPK) phosphatase, DUSP1, in feedback control of inflammatory gene expression and repression by dexamethasone. Shah S, et al. J Biol Chem, 2014 May 9. PMID 24692548
3. Obstructive sleep apnea and intermittent hypoxia increase expression of dual specificity phosphatase 1. Hoffmann MS, et al. Atherosclerosis, 2013 Dec. PMID 24267255 Free PMC Article
4. Mitogen-activated protein kinase phosphatase 1 disrupts proinflammatory protein synthesis in endotoxin-adapted monocytes. Brudecki L, et al. Clin Vaccine Immunol, 2013 Sep. PMID 23825193 Free PMC Article
5. Kaposi’s sarcoma-associated herpesvirus suppression of DUSP1 facilitates cellular pathogenesis following de novo infection. Qin Z, et al. J Virol, 2013 Jan. PMID 23097457 Free PMC Article
6. Oxidative stress and heat shock induce a human gene encoding a protein-tyrosine phosphatase.
   Keyes S.M., Emslie E.A.
   Nature 359:644-647(1992) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Foreskin.
7. Isolation and characterization of a human dual specificity protein-tyrosine phosphatase gene.
   Kwak S.P., Hakes D.J., Martell K.J., Dixon J.E.
   J. Biol. Chem. 269:3596-3604(1994) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
8. NIEHS SNPs program
   Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS THR-56 AND HIS-187.
9. Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R.
   , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases

   Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

10. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.