Phospho-MLK1/2 (T312/266) Polyclonal Antibody, 100µg, (ATB-P1050)

Description

Background:

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade through the phosphorylation of MAP2K4/MKK4 and MAP2K7/MKK7 which in turn activate the JNKs. The MKK/JNK signaling pathway regulates stress response via activator protein-1 (JUN) and GATA4 transcription factors. Plays also a role in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis.

Product datasheet:

References:

1. Phosphoregulation of mixed-lineage kinase 1 activity by multiple phosphorylation in the activation loop. Durkin JT, et al. Biochemistry, 2004 Dec 28. PMID 15610029
2. Complete nucleotide sequence, expression, and chromosomal localisation of human mixed-lineage kinase 2. Dorow DS, et al. Eur J Biochem, 1995 Dec 1. PMID 8536694
3. Identification of a new family of human epithelial protein kinases containing two leucine/isoleucine-zipper domains. Dorow DS, et al. Eur J Biochem, 1993 Apr 15. PMID 8477742
4. Targeted genetic dependency screen facilitates identification of actionable mutations in FGFR4, MAP3K9, and PAK5 in lung cancer. Fawdar S, et al. Proc Natl Acad Sci U S A, 2013 Jul 23. PMID 23836671 Free PMC Article
5. Frequent somatic mutations in MAP3K5 and MAP3K9 in metastatic melanoma identified by exome sequencing. Stark MS, et al. Nat Genet, 2011 Dec 25. PMID 22197930 Free PMC Article
6. Phosphoregulation of mixed-lineage kinase 1 activity by multiple phosphorylation in the activation loop.
   Durkin J.T., Holskin B.P., Kopec K.K., Reed M.S., Spais C.M., Steffy B.M., Gessner G., Angeles T.S., Pohl J., Ator M.A., Meyer S.L.
   Biochemistry 43:16348-16355(2004) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, ENZYME REGULATION, PHOSPHORYLATION AT THR-304; THR-305; SER-308 AND THR-312, MUTAGENESIS OF LYS-171; THR-304; THR-305; SER-308 AND THR-312.
7. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
   The MGC Project Team
   Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
8. cDNA sequence and gene organisation of mixed lineage kinase 1.
   McNee J.J., Dower S.K., Guesdon F.
   Submitted (APR-2000) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [MRNA] OF 53-1104 (ISOFORM 2).
9. Identification of a new family of human epithelial protein kinases containing two leucine/isoleucine-zipper domains.
   Dorow D.S., Devereux L., Dietzsch E., de Kretser T.
   Eur. J. Biochem. 213:701-710(1993) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 142-535, TISSUE SPECIFICITY. Tissue: Colon epithelium.
10. Cep-1347 (KT7515), a semisynthetic inhibitor of the mixed lineage kinase family.
    Maroney A.C., Finn J.P., Connors T.J., Durkin J.T., Angeles T., Gessner G., Xu Z., Meyer S.L., Savage M.J., Greene L.A., Scott R.W., Vaught J.L.
    J. Biol. Chem. 276:25302-25308(2001) [PubMed] [Europe PMC] Cited for: ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, PHOSPHORYLATION OF MAP2K4/MKK4.