Phospho-MSK1 (S212) Polyclonal Antibody, 100µg, (ATB-P0791)

Description

Background:

Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factors RELA, STAT3 and ETV1/ER81, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF and upon glucocorticoid, associates in the cytoplasm with the glucocorticoid receptor NR3C1 and contributes to RELA inhibition and repression of inflammatory gene expression. In skeletal myoblasts is required for phosphorylation of RELA at ‘Ser-276’ during oxidative stress. In erythropoietin-stimulated cells, is necessary for the ‘Ser-727’ phosphorylation of STAT3 and regulation of its transcriptional potential. Phosphorylates ETV1/ER81 at ‘Ser-191’ and ‘Ser-216’, and thereby regulates its ability to stimulate transcription, which may be important during development and breast tumor formation. Directly represses transcription via phosphorylation of ‘Ser-1’ of histone H2A. Phosphorylates ‘Ser-10’ of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate ‘Ser-28’ of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines. Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors. Plays a role in neuronal cell death by mediating the downstream effects of excitotoxic injury.

Product datasheet:

Overview
Product Description	Phospho-MSK1 (S212) Polyclonal Antibody, 100µg, (ATB-P0791)
Image
Species Reactivities	Human,Mouse
Immunogen	Synthesized peptide derived from human MSK1 around the phosphorylation site of S212.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Clonality	Polyclonal

References:

1. Activation of mitogen- and stress-activated kinase 1 is required for proliferation of breast cancer cells in response to estrogens or progestins. Reyes D, et al. Oncogene, 2014 Mar 20. PMID 23604116
2. Histone code pathway involving H3 S28 phosphorylation and K27 acetylation activates transcription and antagonizes polycomb silencing. Lau PN, et al. Proc Natl Acad Sci U S A, 2011 Feb 15. PMID 21282660 Free PMC Article
3. Identification of novel phosphorylation sites in MSK1 by precursor ion scanning MS. McCoy CE, et al. Biochem J, 2007 Mar 15. PMID 17117922 Free PMC Article
4. Mitogen- and stress-activated protein kinase 1 is activated in lesional psoriatic epidermis and regulates the expression of pro-inflammatory cytokines. Funding AT, et al. J Invest Dermatol, 2006 Aug. PMID 16543895
5. Mitogen- and stress-activated protein kinase 1 is critical for interleukin-1-induced, CREB-mediated, c-fos gene expression in keratinocytes. Schiller M, et al. Oncogene, 2006 Jul 27. PMID 16532028
6. Mitogen- and stress-activated protein kinase-1 (MSK1) is directly activated by MAPK and SAPK2/p38, and may mediate activation of CREB.
   Deak M., Clifton A.D., Lucocq J.M., Alessi D.R.
   EMBO J. 17:4426-4441(1998) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, ENZYME REGULATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF ASP-195 AND ASP-565. Tissue: Pancreas1 Publication

7. <p>Manually curated information for which there is published experimental evidence.</p>
10. <p><a href=”/manual/evidences#ECO:0000269″>More…</a></p> Manual assertion based on experiment inⁱ
    Ref.1
    Mitogen- and stress-activated protein kinase-1 (MSK1) is directly activated by MAPK and SAPK2/p38, and may mediate activation of CREB.
    Deak M., Clifton A.D., Lucocq J.M., Alessi D.R.
    EMBO J. 17:4426-4441(1998) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, ENZYME REGULATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF ASP-195 AND ASP-565.