Phospho-MYPT1 (T853) Polyclonal Antibody, 100µg, (ATB-P0662)

Description

Background:

Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009]

Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity.

Product datasheet:

Overview
Product Description	Phospho-MYPT1 (T853) Polyclonal Antibody, 100µg, (ATB-P0662)
Image
Species Reactivities	Human,Mouse,Rat
Immunogen	Synthesized peptide derived from human MYPT1 around the phosphorylation site of T853.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Clonality	Polyclonal

References:

1. Differential phosphorylation of LZ+/LZ- MYPT1 isoforms regulates MLC phosphatase activity. Yuen SL, et al. Arch Biochem Biophys, 2014 Nov 15. PMID 25168281
2. Reconstituted human myosin light chain phosphatase reveals distinct roles of two inhibitory phosphorylation sites of the regulatory subunit, MYPT1. Khasnis M, et al. Biochemistry, 2014 Apr 29. PMID 24712327
3. MYPT1 sustains centromeric cohesion and the spindle-assembly checkpoint. Li J, et al. J Genet Genomics, 2013 Nov 20. PMID 24238611
4. cAMP signaling regulates platelet myosin light chain (MLC) phosphorylation and shape change through targeting the RhoA-Rho kinase-MLC phosphatase signaling pathway. Aburima A, et al. Blood, 2013 Nov 14. PMID 24100445
5. Brain natriuretic peptide protects against hyperresponsiveness of human asthmatic airway smooth muscle via an epithelial cell-dependent mechanism. Calzetta L, et al. Am J Respir Cell Mol Biol, 2014 Mar. PMID 24074453
6. Localization of the gene coding for myosin phosphatase, target subunit 1 (MYPT1) to human chromosome 12q15-q21.
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7. Guo J.H., Chen X.Y., Yu L.
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8. Molecular cloning and functional analysis of a new isoform of human MYPT1.
   Xia D., Kamm K., Stull J.T.
   Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
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   Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC]

   Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5). Tissue: Testis.

10. The finished DNA sequence of human chromosome 12.
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    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].