Phospho-p27 (T187) Polyclonal Antibody, 100 µg, (ATB-P0283)

Description

Background:

Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

Product datasheet:

References:

1. Cloning of p27Kip1, a cyclin-dependent kinase inhibitor and a potential mediator of extracellular antimitogenic signals.
   Polyak K., Lee M.-H., Erdjument-Bromage H., Koff A., Roberts J.M., Tempst P., Massague J.
   Cell 78:59-66(1994) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 28-79 AND 104-152. Tissue: Kidney.
2. Assignment of the human p27Kip1 gene to 12p13 and its analysis in leukemias.
   Pietenpol J.A., Bohlander S.K., Sato Y., Papadopoulos N., Liu B., Friedman C., Trask B.J., Roberts J.M., Kinzler K.W., Rowley J.D.
   Cancer Res. 55:1206-1210(1995) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
3. Cloning of human full-length CDSs in BD Creator(TM) system donor vector.
   Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
   Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLY-109.
4. NIEHS SNPs program
   Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS TRP-15 AND GLY-109.
5. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
   The MGC Project Team
   Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLY-109. Tissue: Cervix.
6. Ubiquitination of p27 is regulated by Cdk-dependent phosphorylation and trimeric complex formation.
   Montagnoli A., Fiore F., Eytan E., Carrano A.C., Draetta G.F., Hershko A., Pagano M.
   Genes Dev. 13:1181-1189(1999) [PubMed] [Europe PMC] Cited for: UBIQUITINATION, PHOSPHORYLATION AT THR-187.
7. Phosphorylation at serine 10, a major phosphorylation site of p27(Kip1), increases its protein stability.
   Ishida N., Kitagawa M., Hatakeyama S., Nakayama K.
   J. Biol. Chem. 275:25146-25154(2000) [PubMed] [Europe PMC] Cited for: PHOSPHORYLATION AT SER-10, FUNCTION.
8. A growth factor-dependent nuclear kinase phosphorylates p27(Kip1) and regulates cell cycle progression.
   Boehm M., Yoshimoto T., Crook M.F., Nallamshetty S., True A., Nabel G.J., Nabel E.G.
   EMBO J. 21:3390-3401(2002) [PubMed] [Europe PMC] Cited for: INTERACTION WITH UHMK1, PHOSPHORYLATION AT SER-10, SUBCELLULAR LOCATION, MUTAGENESIS OF SER-10 AND THR-187.
9. Akt-dependent phosphorylation of p27Kip1 promotes binding to 14-3-3 and cytoplasmic localization.
   Fujita N., Sato S., Katayama K., Tsuruo T.
   J. Biol. Chem. 277:28706-28713(2002) [PubMed] [Europe PMC] Cited for: PHOSPHORYLATION AT SER-10; THR-187 AND THR-198, INTERACTION WITH AKT1 AND YWHAQ, SUBCELLULAR LOCATION, MUTAGENESIS OF SER-10; THR-157; THR-187 AND THR-198.
10. Cytoplasmic relocalization and inhibition of the cyclin-dependent kinase inhibitor p27(Kip1) by PKB/Akt-mediated phosphorylation in breast cancer.
    Viglietto G., Motti M.L., Bruni P., Melillo R.M., D’Alessio A., Califano D., Vinci F., Chiappetta G., Tsichlis P., Bellacosa A., Fusco A., Santoro M.
    Nat. Med. 8:1136-1144(2002) [PubMed] [Europe PMC] Cited for: PHOSPHORYLATION AT THR-157, SUBCELLULAR LOCATION, ASSOCIATION WITH BREAST CANCER, MUTAGENESIS OF THR-157.