Phospho-PEA-15 (S116) Polyclonal Antibody, 100µg, (ATB-P0669)

Description

Background:

This gene encodes a death effector domain-containing protein that functions as a negative regulator of apoptosis. The encoded protein is an endogenous substrate for protein kinase C. This protein is also overexpressed in type 2 diabetes mellitus, where it may contribute to insulin resistance in glucose uptake. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Blocks Ras-mediated inhibition of integrin activation and modulates the ERK MAP kinase cascade. Inhibits RPS6KA3 activities by retaining it in the cytoplasm (By similarity). Inhibits both TNFRSF6- and TNFRSF1A-mediated CASP8 activity and apoptosis. Regulates glucose transport by controlling both the content of SLC2A1 glucose transporters on the plasma membrane and the insulin-dependent trafficking of SLC2A4 from the cell interior to the surface.

Product datasheet:

Overview
Product Description	Phospho-PEA-15 (S116) Polyclonal Antibody, 100µg, (ATB-P0669)
Image
Species Reactivities	Human,Mouse,Rat,Monkey
Immunogen	Synthesized peptide derived from human PEA-15 around the phosphorylation site of S116.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Clonality	Polyclonal

References:

1. PEA15 regulates the DNA damage-induced cell cycle checkpoint and oncogene-directed transformation. Nagarajan A, et al. Mol Cell Biol, 2014 Jun. PMID 24710276 Free PMC Article
2. Phosphorylation-regulated degradation of the tumor-suppressor form of PED by chaperone-mediated autophagy in lung cancer cells. Quintavalle C, et al. J Cell Physiol, 2014 Oct. PMID 24477641 Free PMC Article
3. Reactive astrocytes associated with plaques in TgCRND8 mouse brain and in human Alzheimer brain express phosphoprotein enriched in astrocytes (PEA-15). Thomason LA, et al. FEBS Lett, 2013 Aug 2. PMID 23792157
4. Molecular basis of the PED/PEA15 interaction with the C-terminal fragment of phospholipase D1 revealed by NMR spectroscopy. Farina B, et al. Biochim Biophys Acta, 2013 Aug. PMID 23608947
5. The PEA-15/PED protein regulates cellular survival and invasiveness in colorectal carcinomas. Funke V, et al. Cancer Lett, 2013 Jul 28. PMID 23481023
6. The major astrocytic phosphoprotein PEA-15 is encoded by two mRNAs conserved on their full length in mouse and human.
   Estelles A., Yokoyama M., Nothias F., Vincent J.-D., Glowinski J., Vernier P., Chneiweiss H.
   J. Biol. Chem. 271:14800-14806(1996) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Brain.
7. PED/PEA-15 gene controls glucose transport and is overexpressed in type 2 diabetes mellitus.
   Condorelli G., Vigliotta G., Iavarone C., Caruso M., Tocchetti C.G., Andreozzi F., Cafieri A., Tecce M.F., Formisano P., Beguinot L., Beguinot F.
   EMBO J. 17:3858-3866(1998) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY. Tissue: Heart.
8. Molecular characterization of the human PEA15 gene on 1q21-q22 and association with type 2 diabetes mellitus in Pima Indians.
   Wolford J.K., Bogardus C., Ossowski V., Prochazka M.
   Gene 241:143-148(2000) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
9. Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R.
   , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases

   Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

10. Cloning of human full-length CDSs in BD Creator(TM) system donor vector.
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).