Phospho-Rac GAP1 (S387) Polyclonal Antibody, 100µg, (ATB-P0933)

Description

Background:

The protein encoded by this gene belongs to the GTPase-activating protein (GAP) family. GAPs bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, GAPs negatively regulate Rho-mediated signals. This protein plays a regulatory role in initiation of cytokinesis, controlling cell growth and differentiation of hematopoietic cells, regulating spermatogenesis, and in neuronal proliferation. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2011]

Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Required for proper attachment of the midbody to the cell membrane during cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells.

Product datasheet:

Overview
Product Description	Phospho-Rac GAP1 (S387) Polyclonal Antibody, 100µg, (ATB-P0933)
Image
Species Reactivities	Human,Mouse,Rat,Monkey
Immunogen	Synthesized peptide derived from human Rac GAP1 around the phosphorylation site of S387.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Clonality	Polyclonal

References:

1. Crystal structure of GTPase-activating domain from human MgcRacGAP. Matsuura A, et al. Biochem Biophys Res Commun, 2013 Jun 7. PMID 23665020
2. Expression of RACGAP1 in high grade meningiomas: a potential role in cancer progression. Ke HL, et al. J Neurooncol, 2013 Jun. PMID 23525949
3. Centralspindlin links the mitotic spindle to the plasma membrane during cytokinesis. Lekomtsev S, et al. Nature, 2012 Dec 13. PMID 23235882
4. Upregulation of Rac GTPase-activating protein 1 is significantly associated with the early recurrence of human hepatocellular carcinoma. Wang SM, et al. Clin Cancer Res, 2011 Sep 15. PMID 21825042
5. Sphingosine 1-phosphate-induced mobilization of intracellular Ca2+ mediates rac activation and adherens junction assembly in endothelial cells. Mehta D, et al. J Biol Chem, 2005 Apr 29. PMID 15728185
6. MgcRacGAP is involved in the control of growth and differentiation of hematopoietic cells.
   Kawashima T., Hirose K., Satoh T., Kaneko A., Ikeda Y., Kaziro Y., Nosaka T., Kitamura T.
   Blood 96:2116-2124(2000) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF ARG-385, INDUCTION.
7. Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs.
   Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B.
   , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A.
   Genome Res. 11:422-435(2001) [PubMed] [Europe PMC]

   Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: TestisImported

8. <p>Manually validated information which has been imported from another database.</p>