Phospho-Rad51 (T309) Polyclonal Antibody, 100µg, (ATB-P1106)

Description

Background:

The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]

Participates in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments. Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.

Product datasheet:

References:

1. Association of polymorphisms in the 5′ untranslated region of RAD51 gene with risk of endometrial cancer in the Polish population. Michalska MM, et al. Arch Gynecol Obstet, 2014 Nov. PMID 24930116 Free PMC Article
2. An increased risk of ovarian cancer associated with polymorphism in BRCC5 gene in Caucasian populations. Liang H, et al. Tumour Biol, 2014 Sep. PMID 24927671
3. RAD51 G135C gene polymorphism and risk of colorectal cancer in Kashmir. Nissar S, et al. Eur J Cancer Prev, 2014 Jul. PMID 24871558
4. Interplay of Rad51 with NF-κB pathway stimulates expression of HIV-1. Kaminski R, et al. PLoS One, 2014. PMID 24847939 Free PMC Article
5. Rad51 supports triple negative breast cancer metastasis. Wiegmans AP, et al. Oncotarget, 2014 May 30. PMID 24811120 Free PMC Article
6. Cloning of human, mouse and fission yeast recombination genes homologous to RAD51 and recA.
   Shinohara A., Ogawa H., Matsuda Y., Ushio N., Ikeo K., Ogawa T.
   Nat. Genet. 4:239-243(1993) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
7. Cloning and sequence of the human RecA-like gene cDNA.
   Yoshimura Y., Morita T., Yamamoto A., Matsushiro A.
   Nucleic Acids Res. 21:1665-1665(1993) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Testis.
8. Characterization of the human Rad51 genomic locus and examination of tumors with 15q14-15 loss of heterozygosity (LOH).
   Schmutte C., Tombline G., Rhiem K., Sadoff M.M., Schmutzler R., von Deimling A., Fishel R.
   Cancer Res. 59:4564-4569(1999) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
9. A single nucleotide polymorphism in the 5′ untranslated region of RAD51 and risk of cancer among BRCA1/2 mutation carriers.
   Wang W.W., Spurdle A.B., Kolachana P., Bove B., Modan B., Ebbers S.M., Suthers G., Tucker M.A., Kaufman D.J., Doody M.M., Tarone R.E., Daly M., Levavi H., Pierce H., Chetrit A., Yechezkel G.H., Chenevix-Trench G., Offit K., Godwin A.K., Struewing J.P.
   Cancer Epidemiol. Biomarkers Prev. 10:955-960(2001) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
10. Identification of a novel human Rad51 variant that promotes DNA strand exchange.
    Park J.Y., Yoo H.W., Kim B.R., Park R., Choi S.Y., Kim Y.
    Nucleic Acids Res. 36:3226-3234(2008) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), FUNCTION OF ISOFORMS 1 AND 3, TISSUE SPECIFICITY (ISOFORMS 1 AND 3), SUBCELLULAR LOCATION (ISOFORMS 1 AND 3), MUTAGENESIS (ISOFORM 3).