Phospho-Rb (S780) Polyclonal Antibody, 100µg, (ATB-P0240)

Description

Background:

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]

Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 ‘Lys-20’ trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1’s activity.

Product datasheet:

Overview
Product Description	Phospho-Rb (S780) Polyclonal Antibody, 100µg, (ATB-P0240)
Image
Species Reactivities	Human,Mouse,Rat
Immunogen	Synthesized peptide derived from human Rb around the phosphorylation site of S780.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Clonality	Polyclonal

References:

1. Next-generation sequencing of salivary high-grade neuroendocrine carcinomas identifies alterations in RB1 and the mTOR pathway. Goyal B, et al. Exp Mol Pathol, 2014 Dec. PMID 25446844
2. Rb suppresses human cone-precursor-derived retinoblastoma tumours. Xu XL, et al. Nature, 2014 Oct 16. PMID 25252974
3. Reduced expression of the retinoblastoma protein shows that the related signaling pathway is essential for mediating the antineoplastic activity of erufosine. Zaharieva MM, et al. PLoS One, 2014. PMID 24987858 Free PMC Article
4. Deregulation of RB1 expression by loss of imprinting in human hepatocellular carcinoma. Anwar SL, et al. J Pathol, 2014 Aug. PMID 24838394
5. Genetic testing in Tunisian families with heritable retinoblastoma using a low cost approach permits accurate risk prediction in relatives and reveals incomplete penetrance in adults. Ayari Jeridi H, et al. Exp Eye Res, 2014 Jul. PMID 24810223
6. The retinoblastoma susceptibility gene encodes a nuclear phosphoprotein associated with DNA binding activity.
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   Nature 329:642-645(1987) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
7. Human retinoblastoma susceptibility gene: cloning, identification, and sequence.
   Lee W.-H., Bookstein R., Hong F.D., Young L.-J., Shew J.-Y., Lee E.Y.-H.P.
   Science 235:1394-1399(1987) [PubMed] [Europe PMC] Cited for: SEQUENCE REVISION.
8. Deletions of a DNA sequence in retinoblastomas and mesenchymal tumors: organization of the sequence and its encoded protein.
   Friend S.H., Horowitz J.M., Gerber M.R., Wang X.-F., Bogenmann E., Li F.P., Weinberg R.A.
   Proc. Natl. Acad. Sci. U.S.A. 84:9059-9063(1987) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
9. Structure and partial genomic sequence of the human retinoblastoma susceptibility gene.
   McGee T.L., Yandell D.W., Dryja T.P.
   Gene 80:119-128(1989) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
10. Detection of heterozygous mutations in the RB1 gene in retinoblastoma patients using single-strand conformation polymorphism analysis and polymerase chain reaction sequencing.
    Hogg A., Onadim Z., Baird P.N., Cowell J.K.
    Oncogene 7:1445-1451(1992) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Carcinoma.