Phospho-RUNX1 (S249) Polyclonal Antibody, 100µg, (ATB-P0293)

Description

Background:

Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

CBF binds to the core site, 5′-PYGPYGGT-3′, of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits KAT6B-dependent transcriptional activation.

Product datasheet:

Overview
Product Description	Phospho-RUNX1 (S249) Polyclonal Antibody, 100µg, (ATB-P0293)
Image
Species Reactivities	Human,Mouse,Rat
Immunogen	Synthesized peptide derived from human RUNX1 around the phosphorylation site of S249.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Clonality	Polyclonal

References:

1. Frequent coexistence of RAS mutations in RUNX1-mutated acute myeloid leukemia in Arab Asian children. Al-Kzayer LF, et al. Pediatr Blood Cancer, 2014 Nov. PMID 25066867
2. Expression of RUNX1 correlates with poor patient prognosis in triple negative breast cancer. Ferrari N, et al. PLoS One, 2014. PMID 24967588 Free PMC Article
3. RUNX1 point mutations potentially identify a subset of early immature T-cell acute lymphoblastic leukaemia that may originate from differentiated T-cells. Mok MM, et al. Gene, 2014 Jul 15. PMID 24792891
4. An intragenic long noncoding RNA interacts epigenetically with the RUNX1 promoter and enhancer chromatin DNA in hematopoietic malignancies. Wang H, et al. Int J Cancer, 2014 Dec 15. PMID 24752773
5. Downregulation of RUNX1/CBFβ by MLL fusion proteins enhances hematopoietic stem cell self-renewal. Zhao X, et al. Blood, 2014 Mar 13. PMID 24449215
6. Ahn M.-Y., Bae S.C., Zhang Y.W., Shigesada K., Ito Y.
   Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [MRNA].
7. t(8;21) breakpoints on chromosome 21 in acute myeloid leukemia are clustered within a limited region of a single gene, AML1.
   Miyoshi H., Shimizu K., Kozu T., Maseki N., Kaneko Y., Ohki M.
   Proc. Natl. Acad. Sci. U.S.A. 88:10431-10434(1991) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM AML-1C). Tissue: Leukocyte.
8. AML1 fusion transcripts in t(3;21) positive leukemia: evidence of molecular heterogeneity and usage of splicing sites frequently involved in the generation of normal AML1 transcripts.
   Sacchi N., Nisson P.E., Watkins P.C., Faustinella F., Wijsman J., Hagemeijer A.
   Genes Chromosomes Cancer 11:226-236(1994) [PubMed] [Europe PMC] Cited for: ALTERNATIVE PRODUCTS, CHROMOSOMAL TRANSLOCATION WITH EAP.
9. Involvement of the AML1 gene in the t(3;21) in therapy-related leukemia and in chronic myeloid leukemia in blast crisis.
   Nucifora G., Birn D.J., Espinosa R. III, Erickson P., Lebeau M.M., Roulston D., McKeithan T.W., Drabkin H., Rowley J.D.
   Blood 81:2728-2734(1993) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM AML-1E), CHROMOSOMAL TRANSLOCATION WITH ETO.
10. AML1, AML2, and AML3, the human members of the runt domain gene-family: cDNA structure, expression, and chromosomal localization.
    Levanon D., Negreanu V., Bernstein Y., Bar-Am I., Avivi L., Groner Y.
    Genomics 23:425-432(1994) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM AML-1A). Tissue: Monocyte.