Phospho-TACE (T735) Polyclonal Antibody, 100µg, (ATB-P0335)

Description

Background:

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway. [provided by RefSeq, Jul 2008]

Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). Plays a role in the proteolytic processing of ACE2.

Product datasheet:

Overview
Product Description	Phospho-TACE (T735) Polyclonal Antibody, 100µg, (ATB-P0335)
Image
Species Reactivities	Human,Mouse,Rat
Immunogen	Synthesized peptide derived from human TACE around the phosphorylation site of T735.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Clonality	Polyclonal

References:

1. Senescence-associated release of transmembrane proteins involves proteolytic processing by ADAM17 and microvesicle shedding. Effenberger T, et al. FASEB J, 2014 Nov. PMID 25077560
2. Insights into desmosome biology from inherited human skin disease and cardiocutaneous syndromes. Nitoiu D, et al. Cell Commun Adhes, 2014 Jun. PMID 24738885
3. ADAM17 silencing suppresses the migration and invasion of non-small cell lung cancer. Lv X, et al. Mol Med Rep, 2014 May. PMID 24626788
4. ADAM17 mediates OSCC development in an orthotopic murine model. Simabuco FM, et al. Mol Cancer, 2014 Feb 5. PMID 24495306 Free PMC Article
5. Glycosylation of a disintegrin and metalloprotease 17 affects its activity and inhibition. Chavaroche A, et al. Anal Biochem, 2014 Mar 15. PMID 24361716
6. Cloning of a disintegrin metalloproteinase that processes precursor tumour-necrosis factor-alpha.
   Moss M.L., Jin S.-L.C., Milla M.E., Burkhart W., Carter H.L., Chen W.-J., Clay W.C., Didsbury J.R., Hassler D., Hoffman C.R., Kost T.A., Lambert M.H., Leesnitzer M.A., McCauley P., McGeehan G., Mitchell J., Moyer M., Pahel G.
   , Rocque W., Overton L.K., Schoenen F., Seaton T., Su J.-L., Warner J., Willard D., Becherer J.D.
   Nature 385:733-736(1997) [PubMed] [Europe PMC]

   Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), PARTIAL PROTEIN SEQUENCE. Tissue: Leukocyte and Monocyte.

7. A metalloproteinase disintegrin that releases tumour-necrosis factor-alpha from cells.
   Black R.A., Rauch C.T., Kozlosky C.J., Peschon J.J., Slack J.L., Wolfson M.F., Castner B.J., Stocking K.L., Reddy P., Srinivasan S., Nelson N., Bioani N., Schooley K.A., Gerhart M., Davis R., Fitzner J.N., Johnson R.S., Paxton R.J., March C.J., Cerretti D.P.
   Nature 385:729-733(1997) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B).
8. TNF-alpha convertase enzyme from human arthritis-affected cartilage: isolation of cDNA by differential display, expression of the active enzyme, and regulation of TNF-alpha.
   Patel I.R., Attur M.G., Patel R.N., Stuchin S.A., Abagyan R.A., Abramson S.B., Amin A.R.
   J. Immunol. 160:4570-4579(1998) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A). Tissue: Cartilage.
9. Extracellular signal-regulated kinase phosphorylates tumor necrosis factor alpha-converting enzyme at threonine 735: a potential role in regulated shedding.
   Diaz-Rodriguez E., Montero J.C., Esparis-Ogando A., Yuste L., Pandiella A.
   Mol. Biol. Cell 13:2031-2044(2002) [PubMed] [Europe PMC] Cited for: PHOSPHORYLATION AT THR-735.
10. Tumor necrosis factor-alpha converting enzyme/ADAM 17 mediates MUC1 shedding.
    Thathiah A., Blobel C.P., Carson D.D.
    J. Biol. Chem. 278:3386-3394(2003) [PubMed] [Europe PMC] Cited for: INTERACTION WITH MUC1, FUNCTION.