Phospho-Tau (S516/199) Polyclonal Antibody, 100µg, (ATB-P0498)

Description

Background:

This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer’s disease, Pick’s disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq, Jul 2008]

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Product datasheet:

Overview
Product Description	Phospho-Tau (S516/199) Polyclonal Antibody, 100µg, (ATB-P0498)
Image
Species Reactivities	Human,Mouse,Rat
Immunogen	Synthesized peptide derived from human Tau around the phosphorylation site of S516/199.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Clonality	Polyclonal

References:

1. Resonance Raman spectroscopic measurements delineate the structural changes that occur during tau fibril formation. Ramachandran G, et al. Biochemistry, 2014 Oct 21. PMID 25284680
2. Tau binds to lipid membrane surfaces via short amphipathic helices located in its microtubule-binding repeats. Georgieva ER, et al. Biophys J, 2014 Sep 16. PMID 25229151
3. Serum-induced neurite retraction in CAD cells–involvement of an ATP-actin retractile system and the lack of microtubule-associated proteins. Chesta ME, et al. FEBS J, 2014 Nov. PMID 25112570
4. The role of hippocampal tau protein phosphorylation in isoflurane-induced cognitive dysfunction in transgenic APP695 mice. Li C, et al. Anesth Analg, 2014 Aug. PMID 24977637 Free PMC Article
5. Tau pathology does not affect experience-driven single-neuron and network-wide Arc/Arg3.1 responses. Rudinskiy N, et al. Acta Neuropathol Commun, 2014 Jun 10. PMID 24915991 Free PMC Article
6. Cloning and sequencing of the cDNA encoding a core protein of the paired helical filament of Alzheimer disease: identification as the microtubule-associated protein tau.
   Goedert M., Wischik C., Crowther R., Walker J., Klug A.
   Proc. Natl. Acad. Sci. U.S.A. 85:4051-4055(1988) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM FETAL-TAU). Tissue: Brain.
7. Cloning and sequencing of the cDNA encoding an isoform of microtubule-associated protein tau containing four tandem repeats: differential expression of tau protein mRNAs in human brain.
   Goedert M., Spillantini M.G., Potier M.-C., Ulrich J., Crowther R.A.
   EMBO J. 8:393-399(1989) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM TAU-D). Tissue: Brain.
8. The microtubule binding domain of tau protein.
   Lee G., Neve R.L., Kosik K.S.
   Neuron 2:1615-1624(1989) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS TAU-A AND FETAL-TAU). Tissue: Fetal brain.
9. Multiple isoforms of human microtubule-associated protein tau: sequences and localization in neurofibrillary tangles of Alzheimer’s disease.
   Goedert M., Spillantini M.G., Jakes R., Rutherford D., Crowther R.A.
   Neuron 3:519-526(1989) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS TAU-B; TAU-C; TAU-E AND TAU-F), ASSOCIATION WITH ALZHEIMER DISEASE. Tissue: Brain.
10. Structure and novel exons of the human tau gene.
    Andreadis A., Brown W.M., Kosik K.S.
    Biochemistry 31:10626-10633(1992) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS PNS-TAU; FETAL-TAU AND TAU-F), ALTERNATIVE SPLICING, VARIANT HIS-441.