Phospho-WRN (S1141) Polyclonal Antibody, 100µg, (ATB-P0401)

Description

Background:

This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3′ to 5′ DNA helicase activity, and is also a 3′ to 5′ exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging. [provided by RefSeq, Jul 2008]

Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3′->5′ exonuclease activity towards double-stranded DNA with a 5′-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity). Plays a role in double-strand break repair after gamma-irradiation.

Product datasheet:

Overview
Product Description	Phospho-WRN (S1141) Polyclonal Antibody, 100µg, (ATB-P0401)
Image
Species Reactivities	Human
Immunogen	Synthesized peptide derived from human WRN around the phosphorylation site of S1141.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Clonality	Polyclonal

References:

1. Catalytic activities of Werner protein are affected by adduction with 4-hydroxy-2-nonenal. Czerwińska J, et al. Nucleic Acids Res, 2014. PMID 25170083 Free PMC Article
2. Downregulation of the Werner syndrome protein induces a metabolic shift that compromises redox homeostasis and limits proliferation of cancer cells. Li B, et al. Aging Cell, 2014 Apr. PMID 24757718 Free PMC Article
3. WRN loss induces switching of telomerase-independent mechanisms of telomere elongation. Gocha AR, et al. PLoS One, 2014. PMID 24709898 Free PMC Article
4. Werner syndrome helicase has a critical role in DNA damage responses in the absence of a functional fanconi anemia pathway. Aggarwal M, et al. Cancer Res, 2013 Sep 1. PMID 23867477 Free PMC Article
5. Partial lipodystrophy with severe insulin resistance and adult progeria Werner syndrome. Donadille B, et al. Orphanet J Rare Dis, 2013 Jul 12. PMID 23849162 Free PMC Article
6. Positional cloning of the Werner’s syndrome gene.
   Yu C.-E., Oshima J., Fu Y.-H., Wijsman E.M., Hisama F., Alisch R., Matthews S., Nakura J., Miki T., Ouais S., Martin G.M., Mulligan J., Schellenberg G.D.
   Science 272:258-262(1996) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT PHE-1074.
7. Epigenetic inactivation of the premature aging Werner syndrome gene in human cancer.
   Agrelo R., Cheng W.H., Setien F., Ropero S., Espada J., Fraga M.F., Herranz M., Paz M.F., Sanchez-Cespedes M., Artiga M.J., Guerrero D., Castells A., von Kobbe C., Bohr V.A., Esteller M.
   Proc. Natl. Acad. Sci. U.S.A. 103:8822-8827(2006) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT PHE-1074.
8. Genomic structure of the human Werner’s gene and cloning of the mouse homolog.
   Paeper B.W., Gayle M., Brady W., Swartz A., Gillett L.A., Alisch R.S., Mulligan J., Galas D., Fu Y.-H.
   Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT PHE-1074.
9. NIEHS SNPs program
   Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ILE-114; LYS-343; ILE-387; SER-533; CYS-612; PHE-708; CYS-834; SER-912; LEU-1079; ALA-1133; ILE-1339 AND ARG-1367.
10. DNA sequence and analysis of human chromosome 8.
    Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T.
    , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O’Leary S.B., O’Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
    Nature 439:331-335(2006) [PubMed] [Europe PMC]

    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].