Acetyl-EF-1 α1/2 (K41) Polyclonal Antibody, 100ug, (ATB-K0028)

Description

Background:

This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq, Jul 2008]

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.

Product datasheet:

Overview
Product Description	Acetyl-EF-1 α1/2 (K41) Polyclonal Antibody, 100ug, (ATB-K0028)
Image
Species Reactivities	Human, Mouse, Rat
Immunogen	Synthesized peptide derived from the human EF-1 α1/2 around the acetylation site of K41.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Isotype	IGg

References:

1. All human EF1α promoters are not equal: markedly affect gene expression in constructs from different sources. Zheng C, et al. Int J Med Sci, 2014. PMID 24688302 Free PMC Article
2. Effects of two elongation factor 1A isoforms on the formation of gephyrin clusters at inhibitory synapses in hippocampal neurons. Becker M, et al. Histochem Cell Biol, 2013 Dec. PMID 23839781
3. [Effect of knocking down eEF1A1 gene on proliferation and apoptosis in Jurkat cells and its mechanisms]. Huang Y, et al. Zhongguo Shi Yan Xue Ye Xue Za Zhi, 2012 Aug. PMID 22931638
4. Eukaryotic translation elongation factor 1A induces anoikis by triggering cell detachment. Itagaki K, et al. J Biol Chem, 2012 May 4. PMID 22399298 Free PMC Article
5. Association of translation factor eEF1A with defective ribosomal products generates a signal for aggresome formation. Meriin AB, et al. J Cell Sci, 2012 Jun 1. PMID 22357952 Free PMC Article
6. The primary structure of the alpha subunit of human elongation factor 1. Structural aspects of guanine-nucleotide-binding sites.
   Brands J.H.G.M., Maassen J.A., van Hemert F.J., Amons R., Moeller W.
   Eur. J. Biochem. 155:167-171(1986) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
7. Isolation and characterization of the human chromosomal gene for polypeptide chain elongation factor-1 alpha.
   Uetsuki T., Naito A., Nagata S., Kaziro Y.
   J. Biol. Chem. 264:5791-5798(1989) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
8. Retropseudogenes constitute the major part of the human elongation factor 1 alpha gene family.
   Madsen H.O., Poulsen K., Dahl O., Clark B.F.C., Hjorth J.P.
   Nucleic Acids Res. 18:1513-1516(1990) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Liver.
9. Postnatal expression of a novel mRNA isoform from the human elongation factor-1a gene.
   Shimazu T., Koike K.
   Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
10. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: B-cell, Bone marrow, Cervix, Colon, Hippocampus, Kidney, Lung, Lymph, Mammary gland, Ovary, Pancreas, Placenta, Testis and Uterus.