Acetyl-Histone H2A (K5) Polyclonal Antibody, 100ug, (ATB-K0002)

Description

Background:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq, Jul 2008]

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.

Product datasheet:

Overview
Product Description	Acetyl-Histone H2A (K5) Polyclonal Antibody, 100ug, (ATB-K0002)
Image
Species Reactivities	Human,Mouse,Rat
Immunogen	Synthesized peptide derived from human Histone H2A around the acetylation site of K5.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Isotype	IGg

References:

1. H2A.Z depletion impairs proliferation and viability but not DNA double-strand breaks repair in human immortalized and tumoral cell lines. Taty-Taty GC, et al. Cell Cycle, 2014. PMID 24240188 Free PMC Article
2. Histone H2A.Z controls a critical chromatin remodeling step required for DNA double-strand break repair. Xu Y, et al. Mol Cell, 2012 Dec 14. PMID 23122415 Free PMC Article
3. Acetylation of H2A.Z is a key epigenetic modification associated with gene deregulation and epigenetic remodeling in cancer. Valdés-Mora F, et al. Genome Res, 2012 Feb. PMID 21788347 Free PMC Article
4. H2A.Z maintenance during mitosis reveals nucleosome shifting on mitotically silenced genes. Kelly TK, et al. Mol Cell, 2010 Sep 24. PMID 20864037 Free PMC Article
5. Reconciling the positive and negative roles of histone H2A.Z in gene transcription. Marques M, et al. Epigenetics, 2010 May 16. PMID 20364108
6. Sequence of cDNAs for mammalian H2A.Z, an evolutionarily diverged but highly conserved basal histone H2A isoprotein species.
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   Nucleic Acids Res. 16:1113-1124(1988) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
7. The human histone H2A.Z gene. Sequence and regulation.
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   J. Biol. Chem. 265:15211-15218(1990) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
8. Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201).
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9. Complete sequencing and characterization of 21,243 full-length human cDNAs.
   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
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   Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC]

   Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Tongue.

10. Generation and annotation of the DNA sequences of human chromosomes 2 and 4.
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
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    Nature 434:724-731(2005) [PubMed] [Europe PMC]

    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].