Acetyl-HNF-3α/β/γ (K264/253/211) Polyclonal Antibody, 100ug, (ATB-K0026)

Description

Background:

This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. [provided by RefSeq, Jul 2008]

Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a ‘pioneer’ factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5′-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3′ (By similarity). Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at ‘Lys-5’ (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity). Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis.

Product datasheet:

Overview
Product Description	Acetyl-HNF-3α/β/γ (K264/253/211) Polyclonal Antibody, 100ug, (ATB-K0026)
Image
Species Reactivities	Human, Mouse, Rat
Immunogen	Synthesized peptide derived from the human HNF-3α/β/γ around the acetylation site of K264/253/211.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Isotype	IGg

References:

1. Switch in FOXA1 status associates with endometrial cancer progression. Tangen IL, et al. PLoS One, 2014. PMID 24849812 Free PMC Article
2. NFI transcription factors interact with FOXA1 to regulate prostate-specific gene expression. Grabowska MM, et al. Mol Endocrinol, 2014 Jun. PMID 24801505
3. A systematic approach identifies FOXA1 as a key factor in the loss of epithelial traits during the epithelial-to-mesenchymal transition in lung cancer. Wang H, et al. BMC Genomics, 2013 Oct 4. PMID 24093963 Free PMC Article
4. Forkhead box protein A1 inhibits the expression of uncoupling protein 2 in hydrogen peroxide-induced A549 cell line. Song L, et al. Cell Stress Chaperones, 2014 Jan. PMID 23625627 Free PMC Article
5. Forkhead-box A1 transcription factor is a novel adverse prognosis marker in human glioma. Wang L, et al. J Clin Neurosci, 2013 May. PMID 23510544
6. Molecular cloning of the forkhead transcription factor HNF-3 alpha from a human pulmonary adenocarcinoma cell line.
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   Biochim. Biophys. Acta 1307:17-20(1996) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS ALA-72; MET-124 INS AND ARG-185. Tissue: Lung.
7. The human HNF-3 genes: cloning, partial sequence and mutation screening in patients with impaired glucose homeostasis.
   Navas M.A., Vaisse C., Boger S., Heimesaat M., Kollee L.A., Stoffel M.
   Hum. Hered. 50:370-381(2000) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ALA-72; THR-83; MET-124 INS; ARG-185 AND ASN-448.
8. Genetic variation in the HNF-3alpha gene.
   Yu L., Takeda J.
   Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
9. Complete sequencing and characterization of 21,243 full-length human cDNAs.
   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
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   Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC]

   Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Mammary gland and Trachea.

10. The DNA sequence and analysis of human chromosome 14.
    Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H.
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    Nature 421:601-607(2003) [PubMed] [Europe PMC]

    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].