Phospho-AR (Y363) Polyclonal Antibody, 100µg, (ATB-P0439)

Description

Background:

The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.

Product datasheet:

Overview
Product Description	Phospho-AR (Y363) Polyclonal Antibody, 100µg, (ATB-P0439)
Image
Species Reactivities	Human,Mouse,Rat
Immunogen	Synthesized peptide derived from human AR around the phosphorylation site of Y363.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Clonality	Polyclonal

References:

1. Androgen receptor splice variants contribute to prostate cancer aggressiveness through induction of EMT and expression of stem cell marker genes. Kong D, et al. Prostate, 2015 Feb. PMID 25307492
2. Mutation analysis of androgen receptor gene: multiple uses for a single test. Shojaei A, et al. Gene, 2014 Dec 1. PMID 25241384
3. AR-V7 and resistance to enzalutamide and abiraterone in prostate cancer. Antonarakis ES, et al. N Engl J Med, 2014 Sep 11. PMID 25184630
4. Increased androgen receptor activity and cell proliferation in aromatase inhibitor-resistant breast carcinoma. Fujii R, et al. J Steroid Biochem Mol Biol, 2014 Oct. PMID 25178713
5. 3-alkoxy-pyrrolo[1,2-b]pyrazolines as selective androgen receptor modulators with ideal physicochemical properties for transdermal administration. Ullrich T, et al. J Med Chem, 2014 Sep 11. PMID 25121964
6. The human androgen receptor: complementary deoxyribonucleic acid cloning, sequence analysis and gene expression in prostate.
   Lubahn D.B., Joseph D.R., Sar M., Tan J., Higgs H.N., Larson R.E., French F.S., Wilson E.M.
   Mol. Endocrinol. 2:1265-1275(1988) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
7. Structural analysis of complementary DNA and amino acid sequences of human and rat androgen receptors.
   Chang C., Kokontis J., Liao S.
   Proc. Natl. Acad. Sci. U.S.A. 85:7211-7215(1988) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Prostate.
8. Characterization and expression of a cDNA encoding the human androgen receptor.
   Tilley W.D., Marcelli M., Wilson J.D., McPhaul M.J.
   Proc. Natl. Acad. Sci. U.S.A. 86:327-331(1989) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Prostate.
9. Sequence of the intron/exon junctions of the coding region of the human androgen receptor gene and identification of a point mutation in a family with complete androgen insensitivity.
   Lubahn D.B., Brown T.R., Simental J.A., Higgs H.N., Migeon C.J., Wilson E.M., French F.S.
   Proc. Natl. Acad. Sci. U.S.A. 86:9534-9538(1989) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT AIS MET-866.
10. Specific region in hormone binding domain is essential for hormone binding and trans-activation by human androgen receptor.
    Govindan M.V.
    Mol. Endocrinol. 4:417-427(1990) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT DEL GLY-465–472-GLY.