Phospho-CD32 (Y292) Polyclonal Antibody, 100µg, (ATB-P0325)

Description

Background:

The protein encoded by this gene is a low affinity receptor for the Fc region of immunoglobulin gamma complexes. The encoded protein is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells. Variations in this gene may increase susceptibilty to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis.

Product datasheet:

Overview
Product Description	Phospho-CD32 (Y292) Polyclonal Antibody, 100µg, (ATB-P0325)
Image
Species Reactivities	Human,Mouse,Rat
Immunogen	Synthesized peptide derived from human CD32 around the phosphorylation site of Y292.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Clonality	Polyclonal

References:

1. Inhibition of FcγR-mediated phagocytosis by IVIg is independent of IgG-Fc sialylation and FcγRIIb in human macrophages. Nagelkerke SQ, et al. Blood, 2014 Dec 11. PMID 25352126
2. Defunctioning polymorphism in the immunoglobulin G inhibitory receptor (FcγRIIB-T/T232) does not impact on kidney transplant or recipient survival. Clatworthy MR, et al. Transplantation, 2014 Aug 15. PMID 25022320 Free PMC Article
3. [Preparation of lentivirus containing human FcγRIIB gene and FcγRIIB expression in HT-1080 cells]. Liu H, et al. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi, 2014 Jun. PMID 24909272
4. Cell contact-dependent priming and Fc interaction with CD32+ immune cells contribute to the TGN1412-triggered cytokine response. Bartholomaeus P, et al. J Immunol, 2014 Mar 1. PMID 24470499
5. [Decreased FcγRIIb1 translocation to lipid raft signaling domains in activated B lymphocytes from patients with systemic lupus erythematosus]. Peng K, et al. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi, 2014 Jan. PMID 24405601
6. Fc-gamma-RIIb nucleotide sequences in SLE and non-SLE humans in vivo derived lymphocytes.
   Ng S., Sinclair N.R.S., Anderson C., Bell D.A., Cairns E.
   Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS IIB1 AND IIB2). Tissue: Lymphocyte.
7. Human IgG Fc receptor (hFcRII; CD32) exists as multiple isoforms in macrophages, lymphocytes and IgG-transporting placental epithelium.
   Stuart S.G., Simister N.E., Clarkson S.B., Kacinski B.M., Shapiro M., Mellman I.
   EMBO J. 8:3657-3666(1989) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM IIB2). Tissue: Placenta.
8. Structure and expression of human IgG FcRII(CD32). Functional heterogeneity is encoded by the alternatively spliced products of multiple genes.
   Brooks D.G., Qiu W.Q., Luster A.D., Ravetch J.V.
   J. Exp. Med. 170:1369-1385(1989) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS IIB1; IIB2 AND IIB3), VARIANT PHE-205.
9. Distribution, inducibility and biological function of the cloned and expressed human beta Fc receptor II.
   Engelhardt W., Geerds C., Frey J.
   Eur. J. Immunol. 20:1367-1377(1990) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM IIB2). Tissue: Placenta.
10. Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201).
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM IIB2).