Phospho-Cdc25C (T48) Polyclonal Antibody, 100µg, (ATB-P0688)

Description

Background:

This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known. [provided by RefSeq, Jul 2008]

Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity.

Product datasheet:

Overview
Product Description	Phospho-Cdc25C (T48) Polyclonal Antibody, 100µg, (ATB-P0688)
Image
Species Reactivities	Human
Immunogen	Synthesized peptide derived from human Cdc25C around the phosphorylation site of T48.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Clonality	Polyclonal

References:

1. Purification and biochemical analysis of catalytically active human cdc25C dual specificity phosphatase. Franckhauser C, et al. Biochimie, 2013 Jul. PMID 23567337
2. Cell division cycle 25 homolog c effects on low-dose hyper-radiosensitivity and induced radioresistance at elevated dosage in A549 cells. Zhao Y, et al. J Radiat Res, 2012 Sep. PMID 22843362 Free PMC Article
3. [Prokaryotic expression and purification of human GST-Cdc25C fusion protein and preliminary detection of its function]. Fan ZY, et al. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi, 2012 Mar. PMID 22394631
4. Protection of CDC25 phosphatases against oxidative stress in breast cancer cells: evaluation of the implication of the thioredoxin system. Beillerot A, et al. Free Radic Res, 2012 May. PMID 22360685
5. A novel synthetic 2-(3-methoxyphenyl)-6,7-methylenedioxoquinolin-4-one arrests the G2/M phase arrest via Cdc25c and induces apoptosis through caspase- and mitochondria-dependent pathways in TSGH8301 human bladder cancer cells. Hsu SC, et al. Int J Oncol, 2012 Mar. PMID 22021033
6. Human homolog of fission yeast cdc25 mitotic inducer is predominantly expressed in G2.
   Sadhu K., Reed B.I., Richardson H., Russell P.
   Proc. Natl. Acad. Sci. U.S.A. 87:5139-5143(1990) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT CYS-70.
7. An additional transcript of the cdc25C gene from A431 cells encodes a functional protein.
   Bureik M., Rief N., Drescher R., Jungbluth A., Montenarh M., Wagner P.
   Int. J. Oncol. 17:1251-1258(2000) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5).
8. NIEHS SNPs program
   Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
9. Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R.
   , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases

   Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

10. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT CYS-70. Tissue: Skin.