Phospho-Cdk9 (T186) Polyclonal Antibody, 100µg, (ATB-P0365)

Description

Background:

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. [provided by RefSeq, Jul 2008]

Protein kinase involved in the regulation of transcription. Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A, SUPT5H and RDBP. This complex is inactive when in the 7SK snRNP complex form. Phosphorylates EP300, MYOD1, RPB1/POLR2A and AR, and the negative elongation factors DSIF and NELF. Regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors (e.g. TNF-inducible RELA/p65 activation and IL-6-inducible STAT3 signaling). Promotes RNA synthesis in genetic programs for cell growth, differentiation and viral pathogenesis. P-TEFb is also involved in cotranscriptional histone modification, mRNA processing and mRNA export. Modulates a complex network of chromatin modifications including histone H2B monoubiquitination (H2Bub1), H3 lysine 4 trimethylation (H3K4me3) and H3K36me3; integrates phosphorylation during transcription with chromatin modifications to control co-transcriptional histone mRNA processing. The CDK9/cyclin-K complex has also a kinase activity towards CTD of RNAP II and can substitute for CDK9/cyclin-T P-TEFb in vitro. Replication stress response protein; the CDK9/cyclin-K complex is required for genome integrity maintenance, by promoting cell cycle recovery from replication arrest and limiting single-stranded DNA amount in response to replication stress, thus reducing the breakdown of stalled replication forks and avoiding DNA damage. In addition, probable function in DNA repair of isoform 2 via interaction with KU70/XRCC6. Promotes cardiac myocyte enlargement. RPB1/POLR2A phosphorylation on ‘Ser-2’ in CTD activates transcription. AR phosphorylation modulates AR transcription factor promoter selectivity and cell growth. DSIF and NELF phosphorylation promotes transcription by inhibiting their negative effect. The phosphorylation of MYOD1 enhances its transcriptional activity and thus promotes muscle differentiation.

Product datasheet:

Overview
Product Description	Phospho-Cdk9 (T186) Polyclonal Antibody, 100µg, (ATB-P0365)
Image
Species Reactivities	Human,Mouse,Rat
Immunogen	Synthesized peptide derived from human Cdk9 around the phosphorylation site of T186.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Clonality	Polyclonal

References:

1. A novel CDK9 inhibitor shows potent antitumor efficacy in preclinical hematologic tumor models. Yin T, et al. Mol Cancer Ther, 2014 Jun. PMID 24688048
2. Characterization of molecular and cellular functions of the cyclin-dependent kinase CDK9 using a novel specific inhibitor. Albert TK, et al. Br J Pharmacol, 2014 Jan. PMID 24102143 Free PMC Article
3. Cyclin-dependent kinase 9 links RNA polymerase II transcription to processing of ribosomal RNA. Burger K, et al. J Biol Chem, 2013 Jul 19. PMID 23744076 Free PMC Article
4. Cyclin-dependent kinase 9 activity regulates neutrophil spontaneous apoptosis. Wang K, et al. PLoS One, 2012. PMID 22276149 Free PMC Article
5. A role for CDK9-cyclin K in maintaining genome integrity. Yu DS, et al. Cell Cycle, 2011 Jan 1. PMID 21200140 Free PMC Article
6. PITALRE, a nuclear CDC2-related protein kinase that phosphorylates the retinoblastoma protein in vitro.
   Grana X., de Luca A., Sang N., Fu Y., Claudio P.P., Rosenblatt J., Morgan D.O., Giordano A.
   Proc. Natl. Acad. Sci. U.S.A. 91:3834-3838(1994) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
7. Cloning of a full-length cDNA sequence encoding a cdc2-related protein kinase from human endothelial cells.
   Best J.L., Presky D.H., Swerlick R.A., Burns D.K., Chu W.
   Biochem. Biophys. Res. Commun. 208:562-568(1995) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT ALA-231.
8. Genomic organization and characterization of promoter function of the human CDK9 gene.
   Liu H., Rice A.P.
   Gene 252:51-59(2000) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ALA-231.
9. Cloning of human full-length CDSs in BD Creator(TM) system donor vector.
   Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
   Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
10. NIEHS SNPs program
    Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].