Phospho-Claudin-3 (Y219) Polyclonal Antibody, 100µg, (ATB-P0464)

Description

Background:

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this intronless gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is also a low-affinity receptor for Clostridium perfringens enterotoxin, and shares aa sequence similarity with a putative apoptosis-related protein found in rat. [provided by RefSeq, Jul 2008]

Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.

Product datasheet:

Overview
Product Description	Phospho-Claudin-3 (Y219) Polyclonal Antibody, 100µg, (ATB-P0464)
Image
Species Reactivities	Human
Immunogen	Synthesized peptide derived from human Claudin-3 around the phosphorylation site of Y219.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Clonality	Polyclonal

References:

1. Claudin-3 overexpression increases the malignant potential of colorectal cancer cells: roles of ERK1/2 and PI3K-Akt as modulators of EGFR signaling. de Souza WF, et al. PLoS One, 2013. PMID 24069372 Free PMC Article
2. CLDN3 expression and significance – breast carcinoma versus ovarian carcinoma. Ionescu Popescu C, et al. Rom J Morphol Embryol, 2013. PMID 23529315
3. Down-regulation of claudin-3 is associated with proliferative potential in early gastric cancers. Okugawa T, et al. Dig Dis Sci, 2012 Jun. PMID 22290341
4. Efficient inhibition of ovarian cancer by short hairpin RNA targeting claudin-3. Sun C, et al. Oncol Rep, 2011 Jul. PMID 21519794
5. Claudin-3 acts as a sealing component of the tight junction for ions of either charge and uncharged solutes. Milatz S, et al. Biochim Biophys Acta, 2010 Nov. PMID 20655293
6. Analysis of a human gene homologous to rat ventral prostate.1 protein.
   Peacock R.E., Keen T.J., Inglehearn C.F.
   Genomics 46:443-449(1997) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
7. Clostridium perfringens enterotoxin utilizes two structurally related membrane proteins as functional receptors in vivo.
   Katahira J., Sugiyama H., Inoue N., Horiguchi Y., Matsuda M., Sugimoto N.
   J. Biol. Chem. 272:26652-26658(1997) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
8. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
   The MGC Project Team
   Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon.