Phospho-ERβ (S105) Polyclonal Antibody, 100µg, (ATB-P0553)

Description

Background:

This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq, Jul 2008]

Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.

Product datasheet:

Overview
Product Description	Phospho-ERβ (S105) Polyclonal Antibody, 100µg, (ATB-P0553)
Image
Species Reactivities	Human,Mouse,Rat
Immunogen	Synthesized peptide derived from human ERβ around the phosphorylation site of S105.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Clonality	Polyclonal

References:

1. Gender effect in experimental models of human medulloblastoma: does the estrogen receptor β signaling play a role? Ciucci A, et al. PLoS One, 2014. PMID 25000562 Free PMC Article
2. A phosphotyrosine switch determines the antitumor activity of ERβ. Yuan B, et al. J Clin Invest, 2014 Aug. PMID 24960160 Free PMC Article
3. Polymorphisms in the promoter region of ESR2 gene and susceptibility to ovarian cancer. Schüler S, et al. Gene, 2014 Aug 10. PMID 24881814
4. Lipoxin A4 regulates expression of the estrogen receptor and inhibits 17β-estradiol induced p38 mitogen-activated protein kinase phosphorylation in human endometriotic stromal cells. Chen S, et al. Fertil Steril, 2014 Jul. PMID 24835059
5. Estrogen receptor β2 is inversely correlated with Ki-67 in hyperplastic and noninvasive neoplastic breast lesions. Chantzi NI, et al. J Cancer Res Clin Oncol, 2014 Jun. PMID 24671226
6. The complete primary structure of human estrogen receptor beta (hERbeta) and its heterodimerization with ER alpha in vivo and in vitro.
   Ogawa S., Inoue S., Watanabe T., Hiroi H., Orimo A., Hosoi T., Ouchi Y., Muramatsu M.
   Biochem. Biophys. Res. Commun. 243:122-126(1998) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
7. Cloning and characterization of human estrogen receptor beta isoforms.
   Moore J.T., McKee D.D., Slentz-Kesler K., Moore L.B., Jones S.A., Horne E.L., Su J.-L., Kliewer S.A., Lehmann J.M., Willson T.M.
   Biochem. Biophys. Res. Commun. 247:75-78(1998) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 4), NUCLEOTIDE SEQUENCE [MRNA] OF 409-482 (ISOFORM 5), NUCLEOTIDE SEQUENCE [MRNA] OF 409-472 (ISOFORM 6). Tissue: Mammary gland and Testis.
8. Estrogen receptor-beta mRNA variants in human and murine tissues.
   Lu B., Leygue E., Dotzlaw H., Murphy L.J., Murphy L.C., Watson P.H.
   Mol. Cell. Endocrinol. 138:199-203(1998) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3). Tissue: Ovary.
9. Molecular cloning and characterization of human estrogen receptor beta cx: a potential inhibitor of estrogen action in human.
   Ogawa S., Inoue S., Watanabe T., Orimo A., Hosoi T., Ouchi Y., Muramatsu M.
   Nucleic Acids Res. 26:3505-3512(1998) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), CHARACTERIZATION. Tissue: Testis.
10. Estrogen receptor (ER)-beta isoforms: a key to understanding ER-beta signaling.
    Leung Y.K., Mak P., Hassan S., Ho S.M.
    Proc. Natl. Acad. Sci. U.S.A. 103:13162-13167(2006) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 5 AND 6). Tissue: Prostate.