Phospho-GRF-1 (Y1105) Polyclonal Antibody, 100µg, (ATB-P0635)

Description

Background:

The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids. [provided by RefSeq, Jul 2008]

Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5′-GAGAAAAGAAACTGGAGAAACTC-3′. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP). May also act as a tumor suppressor.

Product datasheet:

Overview
Product Description	Phospho-GRF-1 (Y1105) Polyclonal Antibody, 100µg, (ATB-P0635)
Image
Species Reactivities	Human,Mouse,Rat
Immunogen	Synthesized peptide derived from human GRF-1 around the phosphorylation site of Y1105.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Clonality	Polyclonal

References:

1. p190A RhoGAP is involved in EGFR pathways and promotes proliferation, invasion and migration in lung adenocarcinoma cells. Notsuda H, et al. Int J Oncol, 2013 Nov. PMID 24043274
2. Mitotic down-regulation of p190RhoGAP is required for the successful completion of cytokinesis. Manchinelly SA, et al. J Biol Chem, 2010 Aug 27. PMID 20534586 Free PMC Article
3. p190-A, a human tumor suppressor gene, maps to the chromosomal region 19q13.3 that is reportedly deleted in some gliomas. Tikoo A, et al. Gene, 2000 Oct 17. PMID 11054565
4. Molecular cloning and characterization of a factor that binds the human glucocorticoid receptor gene and represses its expression. LeClerc S, et al. J Biol Chem, 1991 Sep 15. PMID 1894621
5. p120RasGAP is a mediator of rho pathway activation and tumorigenicity in the DLD1 colorectal cancer cell line. Organ SL, et al. PLoS One, 2014. PMID 24465899 Free PMC Article
6. Prediction of the coding sequences of unidentified human genes. XIX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.
   Nagase T., Kikuno R., Hattori A., Kondo Y., Okumura K., Ohara O.
   DNA Res. 7:347-355(2000) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
7. Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones.
   Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.
   DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] Cited for: SEQUENCE REVISION.
8. Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R.
   , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases

   Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

9. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
   The MGC Project Team
   Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
10. p190-A, a human tumor suppressor gene, maps to the chromosomal region 19q13.3 that is reportedly deleted in some gliomas.
    Tikoo A., Czekay S., Viars C., White S., Heath J.K., Arden K., Maruta H.
    Gene 257:23-31(2000) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-1491. Tissue: Mammary cancer.