Phospho-GRK 2 (S29) Polyclonal Antibody, 100µg, (ATB-P0653)

Description

Background:

The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq, Jul 2008]

Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner.

Product datasheet:

Overview
Product Description	Phospho-GRK 2 (S29) Polyclonal Antibody, 100µg, (ATB-P0653)
Image
Species Reactivities	Human,Mouse,Rat
Immunogen	Synthesized peptide derived from human GRK 2 around the phosphorylation site of S29.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Clonality	Polyclonal

References:

1. Mapping the putative G protein-coupled receptor (GPCR) docking site on GPCR kinase 2: insights from intact cell phosphorylation and recruitment assays. Beautrait A, et al. J Biol Chem, 2014 Sep 5. PMID 25049229 Free PMC Article
2. G protein-coupled receptor kinase 2: a link between myocardial contractile function and cardiac metabolism. Woodall MC, et al. Circ Res, 2014 May 9. PMID 24812353
3. G protein-coupled receptor kinase 2 (GRK2) is localized to centrosomes and mediates epidermal growth factor-promoted centrosomal separation. So CH, et al. Mol Biol Cell, 2013 Sep. PMID 23904266 Free PMC Article
4. Reduction of lymphocyte G protein-coupled receptor kinase-2 (GRK2) after exercise training predicts survival in patients with heart failure. Rengo G, et al. Eur J Prev Cardiol, 2014 Jan. PMID 23689525
5. G protein-coupled receptor kinase 2 and group I metabotropic glutamate receptors mediate inflammation-induced sensitization to excitotoxic neurodegeneration. Degos V, et al. Ann Neurol, 2013 May. PMID 23494575 Free PMC Article
6. cDNA cloning and chromosomal localization of the human beta-adrenergic receptor kinase.
   Benovic J.L., Stone W.C., Huebner K., Croce C., Caron M.G., Lefkowitz R.J.
   FEBS Lett. 283:122-126(1991) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
7. High expression of beta-adrenergic receptor kinase in human peripheral blood leukocytes. Isoproterenol and platelet activating factor can induce kinase translocation.
   Chuang T.T., Sallese M., Ambrosini G., Parruti G., de Blasi A.
   J. Biol. Chem. 267:6886-6892(1992) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Blood.
8. Structure of the human gene encoding the beta-adrenergic receptor kinase.
   Penn R.B., Benovic J.L.
   J. Biol. Chem. 269:14924-14930(1994) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
9. NHLBI resequencing and genotyping service (RS&G)
   Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
10. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: PNS and Testis.