Phospho-Lck (Y393) Polyclonal Antibody, 100µg, (ATB-P0572)

Description

Background:

This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described. [provided by RefSeq, Jul 2008]

Non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells. Plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Once stimulated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK. Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production. LCK also contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, which leads to hyperphosphorylation and activation of LCK. Also plays a role in the IL2 receptor-linked signaling pathway that controls the T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates other substrates including RUNX3, PTK2B/PYK2, the microtubule-associated protein MAPT, RHOH or TYROBP.

Product datasheet:

Overview
Product Description	Phospho-Lck (Y393) Polyclonal Antibody, 100µg, (ATB-P0572)
Image
Species Reactivities	Human,Mouse,Rat
Immunogen	Synthesized peptide derived from human Lck around the phosphorylation site of Y393.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Clonality	Polyclonal

References:

1. Lck mediates signal transmission from CD59 to the TCR/CD3 pathway in Jurkat T cells. Lipp AM, et al. PLoS One, 2014. PMID 24454946 Free PMC Article
2. The role of membrane rafts in Lck transport, regulation and signalling in T-cells. Ventimiglia LN, et al. Biochem J, 2013 Sep 1. PMID 23931554
3. LCK is an important mediator of B-cell receptor signaling in chronic lymphocytic leukemia cells. Talab F, et al. Mol Cancer Res, 2013 May. PMID 23505068
4. Conformational states of the kinase Lck regulate clustering in early T cell signaling. Rossy J, et al. Nat Immunol, 2013 Jan. PMID 23202272
5. TCR-induced T cell activation leads to simultaneous phosphorylation at Y505 and Y394 of p56(lck) residues. Nyakeriga AM, et al. Cytometry A, 2012 Sep. PMID 22674786
6. A human T cell-specific cDNA clone (YT16) encodes a protein with extensive homology to a family of protein-tyrosine kinases.
   Koga Y., Caccia N., Toyonaga B., Spolski R., Yanagi Y., Yoshikai Y., Mak T.W.
   Eur. J. Immunol. 16:1643-1646(1986) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
7. Structure and expression of lck transcripts in human lymphoid cells.
   Perlmutter R.M., Marth J.D., Lewis D.B., Peet R., Ziegler S.F., Wilson C.B.
   J. Cell. Biochem. 38:117-126(1988) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
8. Structure of the human lck gene: differences in genomic organisation within src-related genes affect only N-terminal exons.
   Rouer E., van Huynh T., de Souza S.L., Lang M.C., Fischer S., Benarous R.
   Gene 84:105-113(1989) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
9. Oncogenic activation of the Lck protein accompanies translocation of the LCK gene in the human HSB2 T-cell leukemia.
   Wright D.D., Sefton B.M., Kamps M.P.
   Mol. Cell. Biol. 14:2429-2437(1994) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS LEU-28; GLN-LYS-PRO-232 INS; VAL-353 AND LEU-447, PHOSPHORYLATION AT TYR-394 AND TYR-505. Tissue: Leukemia.
10. An aberrant lck mRNA in two human T-cell lines.
    Vogel L.B., Arthur R., Fujita D.J.
    Biochim. Biophys. Acta 1264:168-172(1995) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT), ALTERNATIVE SPLICING. Tissue: Leukemic T-cell.