Phospho-MCM4 (S54) Polyclonal Antibody, 100µg, (ATB-P0354)

Description

Background:

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for ‘once per cell cycle’ DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.

Product datasheet:

Overview
Product Description	Phospho-MCM4 (S54) Polyclonal Antibody, 100µg, (ATB-P0354)
Image
Species Reactivities	Human,Mouse,Monkey
Immunogen	Synthesized peptide derived from human MCM4 around the phosphorylation site of S54.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Clonality	Polyclonal

References:

1. Effect of an MCM4 mutation that causes tumours in mouse on human MCM4/6/7 complex formation. Watanabe E, et al. J Biochem, 2012 Aug. PMID 22668557
2. Minichromosome maintenance (MCM) protein 4 as a marker for proliferation and its clinical and clinicopathological significance in non-small cell lung cancer. Kikuchi J, et al. Lung Cancer, 2011 May. PMID 20884074
3. [Expression and significance of MCM4 in esophageal cancer]. Huang XP, et al. Ai Zheng, 2007 Jan. PMID 17222376
4. MCM4 shares homology to a replication/DNA-binding domain in CTF and is contacted by pRb. Schmitz NM, et al. Biochem Biophys Res Commun, 2004 May 7. PMID 15081408
5. Levels of MCM4 phosphorylation and DNA synthesis in DNA replication block checkpoint control. Ishimi Y, et al. J Struct Biol, 2004 Apr-May. PMID 15037254
6. A human homologue of the yeast replication protein Cdc21. Interactions with other Mcm proteins.
   Musahl C., Schulte D., Burkhart R., Knippers R.
   Eur. J. Biochem. 230:1096-1101(1995) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT MET-650.
7. NIEHS SNPs program
   Submitted (APR-2004) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT GLY-460.
8. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
   The MGC Project Team
   Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT MET-650. Tissue: Testis.
9. The promoters for human DNA-PKcs (PRKDC) and MCM4: divergently transcribed genes located at chromosome 8 band q11.
   Connelly M.A., Zhang H., Kieleczawa J., Anderson C.W.
   Genomics 47:71-83(1998) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-712.
10. MCM4 and PRKDC, human genes encoding proteins MCM4 and DNA-PKcs, are close neighbours located on chromosome 8q12–>q13.
    Ladenburger E.M., Fackelmayer F.O., Hameister H., Knippers R.
    Cytogenet. Cell Genet. 77:268-270(1997) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 1-23.