Phospho-RelB (S552) Polyclonal Antibody, 100µg, (ATB-P0844)

Description

Background:

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric RelB-p50 and RelB-p52 complexes are transcriptional activators. RELB neither associates with DNA nor with RELA/p65 or REL. Stimulates promoter activity in the presence of NFKB2/p49. As a member of the NUPR1/RELB/IER3 survival pathway, may provide pancreatic ductal adenocarcinoma with remarkable resistance to cell stress, such as starvation or gemcitabine treatment. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer in a CRY1/CRY2 independent manner. Increased repression of the heterodimer is seen in the presence of NFKB2/p52.

Product datasheet:

Overview
Product Description	Phospho-RelB (S552) Polyclonal Antibody, 100µg, (ATB-P0844)
Image
Species Reactivities	Human,Mouse
Immunogen	Synthesized peptide derived from human RelB around the phosphorylation site of S552.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Clonality	Polyclonal

References:

1. SUMOylation attenuates the transcriptional activity of the NF-κB subunit RelB. Leidner J, et al. J Cell Biochem, 2014 Aug. PMID 24616021
2. Geldanamycin-mediated inhibition of heat shock protein 90 partially activates dendritic cells, but interferes with their full maturation, accompanied by impaired upregulation of RelB. Trojandt S, et al. J Exp Clin Cancer Res, 2014 Feb 13. PMID 24524692 Free PMC Article
3. Concomitant heterochromatinisation and down-regulation of gene expression unveils epigenetic silencing of RELB in an aggressive subset of chronic lymphocytic leukemia in males. Marteau JB, et al. BMC Med Genomics, 2010 Nov 10. PMID 21062507 Free PMC Article
4. p52-Independent nuclear translocation of RelB promotes LPS-induced attachment. Saito T, et al. Biochem Biophys Res Commun, 2010 Jan 1. PMID 19903458 Free PMC Article
5. Inhibition of RelB by 1,25-dihydroxyvitamin D3 promotes sensitivity of breast cancer cells to radiation. Mineva ND, et al. J Cell Physiol, 2009 Sep. PMID 19373868 Free PMC Article
6. I-Rel: a novel rel-related protein that inhibits NF-kappa B transcriptional activity.
   Ruben S.M., Klement J.F., Maher M., Coleman T.A., Chen C.H., Rosen C.A.
   Genes Dev. 6:745-760(1992) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: T-cell.
7. A transcriptional map in the region of 19q13 derived using direct sequencing and exon trapping.
   Yoshiura K., Murray J.C.
   Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
8. NHLBI resequencing and genotyping service (RS&G)
   Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
9. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
   The MGC Project Team
   Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Blood.
10. RelB, a new Rel family transcription activator that can interact with p50-NF-kappa B.
    Ryseck R.P., Bull P., Takamiya M., Bours V., Siebenlist U., Dobrzanski P., Bravo R.
    Mol. Cell. Biol. 12:674-684(1992) [PubMed] [Europe PMC] Cited for: FUNCTION, IDENTIFICATION IN THE NF-KAPPA-B RELB-P50 COMPLEX.