Phospho-Sgo1 (S14) Polyclonal Antibody, 100µg, (ATB-P1107)

Description

Background:

Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Isoform 3 plays a role in maintaining centriole cohesion involved in controlling spindle pole integrity.

Product datasheet:

References:

1. Shugoshin1 enhances multidrug resistance of gastric cancer cells by regulating MRP1, Bcl-2, and Bax genes. Wang Y, et al. Tumour Biol, 2013 Aug. PMID 23564482
2. Lentivirus-mediated siRNA interference targeting SGO-1 inhibits human NSCLC cell growth. Liu L, et al. Tumour Biol, 2012 Apr. PMID 22161216
3. A novel tumor-derived SGOL1 variant causes abnormal mitosis and unstable chromatid cohesion. Kahyo T, et al. Oncogene, 2011 Nov 3. PMID 21532624
4. Human Sgo1 downregulation leads to chromosomal instability in colorectal cancer. Iwaizumi M, et al. Gut, 2009 Feb. PMID 18635744
5. D-box is required for the degradation of human Shugoshin and chromosome alignment. Fu G, et al. Biochem Biophys Res Commun, 2007 Jun 8. PMID 17448445
6. Shugoshin prevents dissociation of cohesin from centromeres during mitosis in vertebrate cells.
   McGuinness B.E., Hirota T., Kudo N.R., Peters J.-M., Nasmyth K.
   PLoS Biol. 3:433-449(2005) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3; 4; 5; 6 AND 7), FUNCTION, SUBCELLULAR LOCATION.
7. Human SgoL1 inhibits precocious separation of sister centromere in early mitosis.
   Suzuki H., Akiyama N., Tsuji M., Saito S., Eto Y.
   Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3; 4; 5 AND 6).
8. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
   The MGC Project Team
   Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 25-561 (ISOFORM 6). Tissue: Cervix, Lung and Mammary gland.
9. Humoral immunity to human breast cancer: antigen definition and quantitative analysis of mRNA expression.
   Scanlan M.J., Gout I., Gordon C.M., Williamson B., Stockert E., Gure A.O., Jaeger D., Chen Y.-T., Mackay A., O’Hare M.J., Old L.J.
   Cancer Immun. 1:4-4(2001) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 250-527 (ISOFORM 6), TISSUE SPECIFICITY. Tissue: Mammary gland.
10. Human Bub1 defines the persistent cohesion site along the mitotic chromosome by affecting Shugoshin localization.
    Kitajima T.S., Hauf S., Ohsugi M., Yamamoto T., Watanabe Y.
    Curr. Biol. 15:353-359(2005) [PubMed] [Europe PMC] Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.