Phospho-TERT (S824) Polyclonal Antibody, 100µg, (ATB-P0388)

Description

Background:

Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity. [provided by RefSeq, Jul 2008]

Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3′-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5′-TTAGGG-3′. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis.

Product datasheet:

Overview
Product Description	Phospho-TERT (S824) Polyclonal Antibody, 100µg, (ATB-P0388)
Image
Species Reactivities	Human
Immunogen	Synthesized peptide derived from human TERT around the phosphorylation site of S824.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Clonality	Polyclonal

References:

1. Human telomerase reverse transcriptase is a promising target for cancer inhibition in squamous cell carcinomas. Park YJ, et al. Anticancer Res, 2014 Nov. PMID 25368238
2. Homocysteine accelerates senescence of endothelial cells via DNA hypomethylation of human telomerase reverse transcriptase. Zhang D, et al. Arterioscler Thromb Vasc Biol, 2015 Jan. PMID 25359865
3. An antiapoptotic role for telomerase RNA in human immune cells independent of telomere integrity or telomerase enzymatic activity. Gazzaniga FS, et al. Blood, 2014 Dec 11. PMID 25320237 Free PMC Article
4. TERT promoter mutations in gliomas, genetic associations and clinico-pathological correlations. Labussière M, et al. Br J Cancer, 2014 Nov 11. PMID 25314060
5. Frequency of TERT promoter mutations in primary tumors of the liver. Quaas A, et al. Virchows Arch, 2014 Dec. PMID 25267585
6. hEST2, the putative human telomerase catalytic subunit gene, is up-regulated in tumor cells and during immortalization.
   Meyerson M., Counter C.M., Eaton E.N., Ellisen L.W., Steiner P., Caddle S.D., Ziaugra L., Beijersbergen R.L., Davidoff M.J., Liu Q., Bacchetti S., Haber D.A., Weinberg R.A.
   Cell 90:785-795(1997) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
7. Telomerase catalytic subunit homologs from fission yeast and human.
   Nakamura T.M., Morin G.B., Chapman K.B., Weinrich S.L., Andrews W.H., Lingner J., Harley C.B., Cech T.R.
   Science 277:955-959(1997) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Embryonic kidney.
8. Genomic organization and promoter characterization of the gene encoding the human telomerase reverse transcriptase (hTERT).
   Wick M., Zubov D., Hagen G.
   Gene 232:97-106(1999) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
9. Expression profile of a gamma-deletion variant of the human telomerase reverse transcriptase gene.
   Hisatomi H., Ohyashiki K., Ohyashiki J.H., Nagao K., Kanamaru T., Hirata H., Hibi N., Tsukada Y.
   Neoplasia 5:193-197(2003) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
10. Differential alternative splicing expressions of telomerase reverse transcriptase in gastrointestinal cell lines.
    Nagao K., Katsumata K., Aizawa Y., Saito N., Hirata H., Sasaki H., Yamamoto S., Hikiji K., Koiwa T., Hisatomi H.
    Oncol. Rep. 11:127-131(2004) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3 AND 4). Tissue: Stomach cancer.