Phospho-Zyxin (S142) Polyclonal Antibody, 100µg, (ATB-P0481)

Description

Background:

Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform. [provided by RefSeq, Jul 2008]

Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression (By similarity).

Product datasheet:

Overview
Product Description	Phospho-Zyxin (S142) Polyclonal Antibody, 100µg, (ATB-P0481)
Image
Species Reactivities	Human
Immunogen	Synthesized peptide derived from human Zyxin around the phosphorylation site of S142.
Properties
Form	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage Instructions	-20°C/1 year
Clonality	Polyclonal

References:

1. Functional analysis of Zyxin in cell migration and invasive potential of oral squamous cell carcinoma cells. Yamamura M, et al. Int J Oncol, 2013 Mar. PMID 23292068
2. Mechanism of stretch-induced activation of the mechanotransducer zyxin in vascular cells. Suresh Babu S, et al. Sci Signal, 2012 Dec 11. PMID 23233529
3. A zyxin-nectin interaction facilitates zyxin localization to cell-cell adhesions. Gregory Call S, et al. Biochem Biophys Res Commun, 2011 Nov 25. PMID 22056563
4. The LIM domain of zyxin is sufficient for force-induced accumulation of zyxin during cell migration. Uemura A, et al. Biophys J, 2011 Sep 7. PMID 21889443 Free PMC Article
5. MicroRNA-16 targets zyxin and promotes cell motility in human laryngeal carcinoma cell line HEp-2. Wu H, et al. IUBMB Life, 2011 Feb. PMID 21360639
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   J. Biol. Chem. 271:31470-31478(1996) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Umbilical vein.
7. A zyxin-related protein whose synthesis is reduced in virally transformed fibroblasts.
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   Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).

9. Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201).
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    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].